| 瞬时转染Arresten基因对血管内皮细胞迁移的影响 |
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| 引用本文： | 熊俊,宋自芳,卢昕,胡青钢,屈新才,郑启昌. 瞬时转染Arresten基因对血管内皮细胞迁移的影响[J]. 肿瘤, 2005, 25(5): 420-422 |
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| 作者姓名： | 熊俊 宋自芳 卢昕 胡青钢 屈新才 郑启昌 |
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| 作者单位： | 华中科技大学同济医学院协和医院外科,武汉,430022 |
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| 基金项目： | 国家自然科学基金，湖北省科技厅科技攻关项目 |
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| 摘 要： | 目的研究瞬时转染Arresten基因对血管内皮细胞迁移的影响,并为后续动物实验探索最佳体内转染DNA-脂质体比例.方法以不同比例之质粒DNA-Lipofectamine 2000转染肝癌细胞系HepG-2细胞,瞬时转染经RT-PCR证实目的基因已转入细胞内48 h后收集上清,上清液与血管内皮细胞以划痕法共培养,计算迁移率.结果不同比例转染细胞的上清液均可抑制内皮细胞的迁移,以DNA-脂质体比例1:1,抑制率最高.结论瞬时转染Arresten基因可抑制血管内皮细胞的迁移,并为后续动物实验奠定一定的基础.
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| 关 键 词： | 血管生成抑制因子 Arresten基因 转染 内皮,血管 细胞游走抑制 ECV-304细胞 |
| 文章编号： | 1000-7431（2005）05-0420-03 |
| 收稿时间： | 2004-09-20 |
| 修稿时间： | 2004-12-21 |
Inhibition of endothelial cell migration by transient transfection of Arresten gene |
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| XIONG Jun,SONG Zifang,LU Xin,HU Qinggang,QU Xincai,ZHENG Qichang. Inhibition of endothelial cell migration by transient transfection of Arresten gene[J]. Tumor, 2005, 25(5): 420-422 |
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| Authors: | XIONG Jun SONG Zifang LU Xin HU Qinggang QU Xincai ZHENG Qichang |
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| Affiliation: | Department of General Surgery, Xiehe Hosipital, Tongji Medical College, Huazhong Scientific and Technical University , Wuhan 430022, China |
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| Abstract: | Objecitive To study inhibition of endothelial cell migration by transient transfection of Arresten gene and find out the best ratio of DNA and Lipofectamine. Methods HepG-2 cells were transfected by different ratio of plasmid arresten DNA-Lipo-fectamine complex. Successful transfection were confirmed by RT-PCR. Supernatants were collected after 48 hours and those were added into DMEM containing 10% new-born calf serum of endothelial cells. The migration ratio was calculated. Results Supernatants of different ratio of DNA-Lipofectamine inhibited endothelial cells migration and the effect was best when the ratio was 1 : 1. Conclusion Arresten gene could inhibit endothelial cells migration. Foundation of animal experiments were built. |
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| Keywords: | Angiogenesis inhibitors Arresten gene Transfection Endothelium, vascular Cell migration inhibition EVC-304 cells |
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