| Institution: | 1. Department of Imaging Diagnostics, Children’s Clinical Hospital, Medical University of Bialystok, Bialystok, Poland;2. Department of Pediatrics and Nephrology, Medical University of Bialystok, Bialystok, Poland;3. Medical Institute, ?omza State University of Applied Sciences, ?omza, Poland;4. Department of Psychiatry, Medical University of Bialystok, Bialystok, Poland |
| Abstract: | PurposeThe purpose of the study was to determine the effect of age on lysosomal exoglycosidase activities: α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase in healthy children and adolescents.Material and methodsUrine samples were collected from 203 healthy children and adolescents (girls?=?99, boys?=?104), aged six months to 17.9 years. The activities of α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase were determined by colorimetric method and expressed in pKat/μg of creatine (pKat/μg Cr.).ResultsUrinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase activities (pKat/μg Cr.) were the highest in children below 3 years of age in comparison to the remaining age groups. There was a statistically significant negative correlation between urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase (pKat/μg Cr.) and age (r?=??0.36; r?=??0.36; r?=??0.35; r?=??0.35; at p?<?0.0001, respectively). In addition, we constructed the reference values for urinary activity of α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase (pKat/μg Cr.) in percentiles according to age in 3-year intervals.ConclusionsOur study is the first to show reference values for urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase in children and adolescents. |
