Cloning and immunological characterisation ofEchinococcus granulosus ferritin |
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| Authors: | Klaus Ersfeld Philip S Craig |
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| Institution: | (1) Present address: Department of Biological Sciences, University of Salford, M5 4WT Salford, UK;(2) Department of Biochemistry, University of Manchester, Stopford Building, Oxford Road, M13 9PT Manchester, UK |
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| Abstract: | A cDNA clone ofEchinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. The deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin.E. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 Da and a statistical isoelectric point of 7.6. Functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. In vitro-translatedE. granulosus ferritin was tested for its diagnostic potential by immunoprecipitation. The antigenic reactivity exhibited a good potential for the further development ofE. granulosus ferritin as an immunodiagnostic tool for human hydatidosis. |
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