HPLC法测定千柏鼻炎胶囊中大黄酚的含量

目的:建立HPLC法测定千柏鼻炎胶囊中大黄酚的含量.方法:采用Agilent C18柱(4.6 mm×250 mm,5 μm),甲醇-水(88∶12)为流动相,流量1.0 mL·min-1,检测波长254 nm,柱温25 ℃.结果:大黄酚在0.02～0.10 μg·mL-1范围内线性关系良好,r=0.999 8,平均回收率为99.5%,RSD为0.5%.结论:方法灵敏,方便,分离效果良好,适用于该制剂中大黄酚的含量测定.

HPLC Determintaion of Chrysophanol in Qianbai Biyan Capsules

Objective：To establish an HPLC method for determination of chrysophanol in QianbaiBiyan Capsules. Method： The Agi- lent Cls column（4. 6 mm ×250 mm,5μm）was used, and mobile phase was methanol -water（88： 12）. The flow rate was 1.0 mL min-1 , the detection Wavelength was at 254 nm. Result： A good linear range of Chrysophanol was 0. 02 0. 10 μg. The average recovery was 99. 5 % （RSD = 0. 5 % ）. Conclusion： The method is sensitive and accurate. It was adequate to determinte of Chrysophanol in Qianbai Biyan Capsules.