骨小梁Micro—CT检测评价阿仑膦酸钠预防大鼠激素性股骨头坏死的效果

目的探讨阿仑膦酸钠预防大鼠激素性股骨头坏死的疗效。方法将200只sD大鼠随机分成两组，其中实验组（A组）140只，对照组（B组）60只。对实验组大鼠给予连续灌喂阿仑膦酸钠溶液干预，对照组大鼠给予连续灌喂生理盐水，两组大鼠同时给予灌喂阿莫西林预防感染，2周后对所有大鼠均连续腹腔内注射大剂量甲级强的松龙4周。注射结束后2、4、8、12周分别处死等量大鼠进行micro—CT检测和HE染色评估造模成功率。结果计算骨小梁空骨陷窝率得出A组大鼠通过组织病理学检测显示造模成功率约30％，而B组造模成功率达50％。A组大鼠在干预开始后2、4、8、12周股骨头标本行Mirco—CT检查显示骨小梁平均间距分别为0．766、0．761、0．753、0．501um，骨小梁平均数目分别为6．146、6．159、6．194、6．723，骨小梁平均厚度为0．097、0．101、0．109、0．138um，组内2、4、8周取材检测结果无明显统计学意义（F值分别为1．670，452．719，1．737，P值均〉0．05）；但是12周时取材检测结果和前次检测结果之间存在显著差异，结果具有统计学意义（F值分别为26．178，58719．299，23．023，P〈0．05）；B组于12周时micro—CT检测结果提示骨小梁平均间距、平均数目、平均厚度分别为0．765um、6．141、0．093um，与干预组12周时Micro-CT结果均具有显著统计学意义（，值分别为13．498，22．557，52．072，P〈0．05）。结论阿仑膦酸钠预防大鼠早期激素性股骨头坏死具有一定作用，但是预防人体股骨头坏死是否具有相同疗效尚需进行多中心临床研究。

Micro-CT evaluation of alendronate in preventing glucocorticoids-induced osteonecrosis of femoral heads of rats

Objective To study the effects of alendronate in preventing early glucocorticoidsinduced osteonecrosis of femoral head （ONFH） in rats by micro-CT. Methods 200 SD rats were divided into two groups randomly ：140 rats were in the experimental group, and 60 rats were in the control group. The experimental group were injected alendronate solution intraperitoneally and the control group were injected physiological saline intraperitoneally for two weeks. Then all the rats were injected high-dose of methylprednisolone for four weeks continuously. At the 2nd week, the 4th week, the 8th week and the 12th week after intervention, the rats in each group were sacrificed. The samples were stained by hematoxylin and eosin （HE） to calculate the success rates of glucocorticoid-induced osteonecrosis of the femoral head and the trabeculae of the femoral head were scanned by Micro-CT. Results The success rate of glucocorticoid-induced osteonecrosis of the femoral head in the experiment group was 30%, but it was about 50 % in the control group. At the 2nd week, the 4th week, the 8th week, and the 12th week after alendronate intervention beginning, accroding to Micro-CT examination, the average trabecular space of the femoral head in the experimental group was 0.766, 0.761, 0.753, and 0.501 um respectively; the average trabecular number was 6. 146, 6. 159, 6. 194, and 6. 723 respectively ; the average trabecular thickness was 0. 097, 0. 101, 0. 109, and 0. 138 um respectively. The results among the 2nd week, the 4th week, and the 8th week had no significant,statistica/meaning （P 〉 0. 05 ）, but comparing to the 12th week, the results had significant difference （P 〈 0. 05 ）. Comparing to the control group, at the 12th week, the micro-CT results of the average trabecular space, average trabecular number and average trabecular thickness were 0. 765 um, 6. 141 and 0. 093um respectively. These micro-CT results of the two groups had obviously statistical significance （P 〈 0. 05 ）. Conclusions Alendronate plays a certain role in the prevention of osteonecrosis of the femoral head caused by glucocorticoids in the early stage in rats according to the micro-CT results, but whether alendronate has the same effects on osteonecrosis of femoral head in human still needs more multi-center clinical trials.