白癜风毛囊无色素性黑素细胞c—kit的表达

目的探讨毛囊无色素性黑素细胞（amelanotic melanocytes，AMMC）和于细胞因子（stem cell factor，SCF）及其受体c—kit（SCF／c—kit）信号通路在白癜风发病机制中的作用。方法采用苏木精伊红（HE）染色和免疫组织化学方法对31例白癜风皮损头皮组织毛囊和30例正常人头皮组织毛囊进行HMB-45、NKI／beteb、c—kit3种特异性抗体染色，观察毛囊AMMC的形态分布特征及c—kit的表达，并以HPIAS-1000彩色病理图文分析系统半定量分析3种特异性标志物表达的强弱，观察两组之间有无差异。结果白癜风皮损头皮组毛囊AMMC的NKI／beteb染色的平均阳性细胞面积率和平均光密度值与正常头皮组织相比，差异无统计学意义（P〉0．05）；白癜风皮损头皮毛囊组AMMC的c—kit染色平均阳性面积率和平均光密度值明显低于正常头皮组，差异具有统计学意义（P〈0．05）。结论白癜风头部皮损毛囊AMMCckit的表达异常，其可能参与了白癜风的发病过程。

Expression of c-kit on amelanotic melanocyte in follicle from vitiligo patients

Objective To analyse preliminarily the role of amelanotic melanocutes (AMMC) and SCF/c-kit signal pathway in the pathogenesis of vitiligo. Methods Three antibodies such as HMB-45, NKl/beteb and c-kit were used to stain sections from scalps from vitiligo patients and the healthy controls. Results There were no HMB-45 positive cells in outer root sheath(ORS) of follicle. NKI/beteb positive cells were small and located in groups at the middle and lower of outer root sheath with their retraction of the dendrites. They only expressed premelanosomal antigens but not melanosomal antigen such as HMB-45. There were no significant difference of AMMC in quantities between vitiligo patients and normal controls (P>0. 05). The expression of c-kit receptors on AMMC in follicle of depigment-ed scalps from vitiligo patients was lower than that in normal contols (P<0. 05). Conclusion Abnormal c-kit expression in AMMC in the follicle of depigmented scalps may play an important role in the pathogenesis of vitiligo.