| 新一代骨替代材料:以可吸收性珊瑚羟基磷灰石为载体体外培养骨髓基质细胞 |
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| 引用本文： | 曹罡,毛天球,曹军,席庆,陈富林,杨维东,陶凯.新一代骨替代材料:以可吸收性珊瑚羟基磷灰石为载体体外培养骨髓基质细胞[J].中国组织工程研究与临床康复,2002,6(8):1117-1118. |
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| 作者姓名： | 曹罡 毛天球 曹军 席庆 陈富林 杨维东 陶凯 |
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| 作者单位： | 解放军第四军医大学口腔医学院,陕西,西安,710033 |
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| 基金项目： | 国家重点基础研究发展规化项目(973项目)(G1999054308) |
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| 摘 要： | 目的探讨以可吸收性珊瑚羟基磷灰石(Interpore500R)为细胞载体对骨髓基质细胞向成骨细胞分化、表型维持的影响。方法将生长状态良好的人骨髓基质细胞接种于Interpore500R上后行体外培养,通过细胞计数检测附着后的生长增殖特性、镜下观察附着情况及细胞形态,共聚焦显微镜下观察细胞分泌I型胶原情况,测定细胞内碱性磷酸酶含量变化。结果骨髓基质细胞接种后可继续增殖,并表达成骨细胞特性。结论可吸收性珊瑚羟基磷灰石具有促进骨髓基质细胞表达成骨细胞表型、合成细胞外基质的功能,是良好的细胞载体。
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| 关 键 词： | 骨髓基质细胞 成骨细胞 可吸收性 羟基磷灰石 I型胶原 碱性磷酸酶 |
| 文章编号： | 1671-5926(2002)08-1117-02 |
| 修稿时间： | 2002年1月28日 |
Culturing human bone marrow stromal cells on the surface of absorbable coral-hydroxyapatite |
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| CAO Gang,MAO Tian Qiu,CAO Jun,et al..Culturing human bone marrow stromal cells on the surface of absorbable coral-hydroxyapatite[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2002,6(8):1117-1118. |
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| Authors: | CAO Gang MAO Tian Qiu CAO Jun |
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| Institution: | CAO Gang,MAO Tian Qiu,CAO Jun,et al.Department of Oral Maxillofacial Surgery,Qing Du Stomatological Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | Objective To explore the effect of absorbable coral hydroxyapatite (Interpore 500R) used as cell culturing scaffold on the differentiation of human marrow stromal cells. Methods After inoculating the cells onto the surface of Interpore 500R, we surveyed the characteristic of proliferation by cell counting. Using scanning electronic microscope we observed celluar morphology. Staining collagen of I type (immunocytochemistry method) and observing the results under confocal microscope. Measured the content of cellular ALP. Results Human marrow stromal cells went on proliferating and started to differentiate to osteoblast after they attched on the material surface. Conclusion The absorbable coral hydroxyapatite is a good bone tissue engineering scaffold. |
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| Keywords: | marrow stromal cell osteoblast absorbable hydroxyapatite collagen of I type alkaline phosphatase(ALP) |
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