HPLC法测定氯甲霜中氯霉素和甲硝唑的含量

目的：建立高效液相色谱法测定氯甲霜中氯霉素和甲硝唑含量.方法：色谱柱Diamonsil C18（250 mm×4.6 mm,5μm）,流动相为0.1%庚烷磺酸钠溶液（0.1%庚烷磺酸钠溶液500 ml与二甲基甲酰胺5 ml,冰醋酸0.5 ml）-乙腈（80：20）,流速为1.0 ml·min-1,检测波长为278 nm.结果：氯霉素和甲硝唑浓度在30～150μg·ml-1范围内均呈良好的线性关系,r=0.999 6和r=0.998 1 平均回收率分别为101.23%（RSD=0.69%）,99.63%（RSD=0.61%）（n=9）.结论：本方法简便,准确,可用于氯甲霜中氯霉素和甲硝唑的含量测定.

Determination of Chloramphenicol and Metronidazole in Compound Chloramphenicoi Cream by HPLC

Objective： To establish an HPLC method for the determination of chloramphenicol and metronidazole in compound chloramphenicol cream. Method： The Diamonsil C18 （250 mm × 4.6 mm,5 μm） was used, the mobile phase consisted of 0. 1% sodium heptanesulfonate solution （a mixture of 0. 1% sodium heptanesulfonate 500 ml, dimethylformamide 5 ml and acetic acid glacial 0. 5 ml）-acetonitril （80： 20）. The flow rate was 1.0 ml.min-1 ,the detection wavelength was 278 nm. Result： The linear ranges of chloramphenicol and metronidasole both were 30-150 μg. ml - 1 （ r = 0. 999 6 and r = 0. 998 1 ）, the average recoveries （ n = 9） were 101.2% and 99. 6% with RSD 0. 69% and 0. 61% ,respectively. Conclusion： The method is simple and accurate,it can be used for the quality control of compound chloramphenicol cream.