生脉姜黄散对鼻咽癌CNE-2细胞裸鼠移植瘤的放射增敏作用

目的:探讨生脉姜黄散对人鼻咽癌细胞CNE-2裸鼠移植瘤的放射增敏作用及对细胞周期素依赖性激酶6(cyclin-dependent kinase 6,CDK6)蛋白表达的影响,并讨论其增敏作用的相关机制。方法:通过皮下注射肿瘤细胞的方法建立人鼻咽癌裸鼠移植瘤模型,荷瘤鼠随机分为空白组、照射组、生脉姜黄散组、生脉姜黄散联合照射组、顺铂联合照射组,其中生脉姜黄散组和生脉姜黄散联合照射组按照药物高、低剂量(12.87,4.29 g·kg-1)各分为2个亚组。经处理后各组隔日测量移植瘤的最大径,计算肿瘤体积抑制率和抑瘤率。免疫组化、蛋白免疫印迹法检测CDK6蛋白表达,实时荧光定量聚合酶链式反应(Real-time PCR)检测CDK6 mRNA表达。结果:与空白组比较,其余各组肿瘤生长都受到一定程度的抑制,与照射组比较,生脉姜黄散(12.87 g·kg-1)联合照射组、顺铂联合照射组肿瘤生长明显受到抑制(P0.05),肿瘤体积抑制率分别为54.94%,64.9%。与照射组比较,生脉姜黄散(12.87 g·kg-1)联合照射组、顺铂联合照射组CDK6 mRNA和蛋白表达均明显升高(P0.05)。结论:生脉姜黄散对人鼻咽癌裸鼠移植瘤具有放射增敏作用,其增敏作用可能与细胞周期调控因子CDK6相关。

Radiosensitization Effect of Shengmai Jianghuang Powder on Human Nasopharyngeal Carcinoma CNE-2 Cell in Nude Mice

Objective: To explore the radiosensitization effect of Shengmai Jianghuang powder on human nasopharyngeal carcinoma CNE-2 cell in nude mice and the effect on the expressions of cyclin-dependent kinase 6 (CDK6) proteins, in order to explore the mechanism of radiosensitization.Method: The tumor-bearing mice were randomly divided into 7 groups:control group, irradiation group, Shengmai Jianghuang powder group, Shengmai Jianghuang powder and irradiation combined group, cisplatin and irradiation combined group. Shengmai Jianghuang powder group, Shengmai Jianghuang powder and irradiation combined group were divided into high and low-dose subgroups with the concentration of 12.87,4.29 g · kg^-1 respectively. The maximal diameter of the transplanted tumor was measured every other day after treatment, and the tumor volume inhibition rate and the tumor inhibition rate were calculated. The protein expression of CDK6 was detected by immunohistochemistry and Western blot. The mRNA expression of CDK6 was detected by Real-time PCR.Result: Compared with the control group, the tumor growth was inhibited to a certain extent. Compared with the irradiation group, the growth of tumor was significantly inhibited in the high-concentration Shengmai Jianghuang powder and irradiation combined group and the cisplatin and irradiation combined group, with the tumor volume inhibition rates of 54.94% and 64.9%, respectively. Immunohistochemistry, Western blot and Real-time PCR showed significant differences in the expression of CDK6 between the high-concentration Shengmai Jianghuang powder and irradiation combined group and the cisplatin and irradiation combined group (P<0.05).Conclusion: Shengmai Jianghuang powder has a radiosensitizing effect on human nasopharyngeal carcinoma CNE-2 cells in nude mice, which may be related to the cell cycle regulatory factor CDK6.