基于颜色变化的麸炒白术最佳炮制火候的客观量化判别

目的:拟建立麸炒白术最佳炮制火候的客观量化判别方法,为白术炮制工艺的规范化研究及改进该饮片的质量标准提供科学依据,并为其他中药饮片炮制火候判别方法的建立提供参考。方法:基于色度分析原理,引入色度空间参数L*,a*,b*(L*为亮度,a*为红绿色度值,b*为黄蓝色度值)值,采用色彩色差计对麸炒白术饮片颜色进行客观量化。经SPSS21.0统计软件分析,建立麸炒白术不同炮制火候的数学判别模型和双侧90%参考值范围。结果:建立了基于色度空间参数L*,b*的麸炒白术不同炮制火候的数学判别模型(判别符合率100%)及双侧90%参考值范围。麸炒12 min时白术达最佳炮制火候,非标准化典则判别函数式(1)为炮制时间=0.675×L*+0.972×b*-49.365,函数值范围为10.103 6~16.051 4。判别函数式(2)为炮制时间=0.884×L*-0.998×b*-11.277,函数值范围为-6.366 0~0.496 7。双侧90%参考值范围为L*44.127 8~47.661 2和b*30.674 5~34.112 3。结论:基于颜色变化能够实现麸炒白术最佳炮制火候的客观量化判别,后续将探索不同炮制火候的麸炒白术饮片颜色与药效作用之间的相关性。

Objective Quantification Discriminant of Optimum Processing Time of Atractylodis Macrocephalae Rhizoma Stir-fried with Bran Based on Color Change

Objective: To establish an objective and quantitative method for the determination of optimum processing time of Atractylodis Macrocephalae Rhizoma stir-fried with bran,and to provide scientific basis for the standardization of processing technology of Atractylodis Macrocephalae Rhizoma and improving quality standards of this decoction pieces.At the same time,this study can provide reference for establishment of determining processing time of other Chinese medicinal decoction pieces.Method: Based on the principle of colorimetric analysis,the color space parameters of L^*,a^* and b^* (L^* indicates brightness,a^* indicates red and green color value,and b^* indicates yellow and blue color value) were introduced,and CM-5 color difference meter was used for objective quantification of color of Atractylodis Macrocephalae Rhizoma stir-fried with bran.SPSS 21.0 statistical software was used to establish mathematical discrimination model and bilateral 90% reference range of different processing time of this decoction pieces.Result: A mathematical discriminant model based on color space parameters of L^* and b^* for different processing time of Atractylodis Macrocephalae Rhizoma stir-fried with bran was established (the coincidence rate was 100%) and bilateral 90% reference range was determined.The optimum processing time of this decoction pieces was 12 min,discriminant function (1) was processing time=0.675×L^*+0.972×b^*-49.365,the range of function values was 10.103 6-16.051 4;discriminant function (2) was processing time=0.884×L^*-0.998×b^*-11.277,the range of function values was -6.366 0-0.496 7.The bilateral 90% reference range is L^* of 44.127 8-47.661 2 and b^* of 30.674 5-34.112 3.Conclusion: Based on the color change can achieve the objective quantification discriminant of optimum processing time of Atractylodis Macrocephalae Rhizoma stir-fried with bran.