哺乳类松果体内肽类神经分布的免疫组织化学研究

用免疫组织化学方法以光镜和电镜观察了金黄地鼠、大白鼠、沙土鼠松果体内的肽类神经分布,其中应用的第一抗体为降钙素基因相关肽(CGRP)、P物质(SP)、血管活性肠肽(VIP)神经肽Y(NPY)、促生长激素释放抑制激素(SRIH),结果发现,SP免疫反应阳性神经纤维主要分布于松果体实质的周围部,而在松果体实质中心部较缺乏。CGRP免疫反应阳性神经纤维在松果体实质内分布较丰富。在松果体实质周围部的CGR与PSP免疫反应的阳性神经纤维分布几乎重复,电镜观察结果,CGRP免疫反应阳性神经纤维为无髓纤维,与松果体细胞相接触,但无膜特化的结构,VIP免疫反应阳性神经纤维分布于松果体实质内,为无髓纤维,与松果体细胞相接触,但无膜特化的结构。NPY免疫反应阳性神经纤维更丰富地分布于整个松果体实质内,但其实质的周围部分布较密集。NPY免疫反应阳性神经纤维为无髓纤维,其末梢内含有大颗粒小泡和小颗粒小泡,神经末梢与松果体细胞相接触,但无膜特化的结构。SRIH免疫反应阳性神经纤维主要分布于松果体实质的中心部。

Immunohistochemical Studies on the Peptidergic Innervation in the Mammalian Pineal Gland

Peptidergic innervation of the pineal gland of golden hamsters, rats and gerbils was investigated by light and electron microscopic immunohistochemistry using antisera to calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), and somatotropin release inhibiting hormone (SRIH). SP-positive nerve fibers were distributed in the peripheral parenchyma, but was rather scanty in the central parenchyma. CGRP-positive nerve fibers were more numerous and distributed evenly in the pineal gland in golden hamsters. GRP-positive unmyelinated nerve terminals were sometimes closely attached to the pinealcytes, although no membrane specialization was noticed. VIP-positive nerve fibers were mainly distributed in the parenchyma. VIP-positive unmyelinated nerve terminals were sometimes closely attached to the pinealocytes althoug no membrane specialization was noticed, NPY-positive nerve fibers were most abundantly demonstrated in all parts of the pineal gland, but more densely distributed in the peripheral parenchyma than in the center. NPY-positive terminals contained numerious small vesicles. NPY-positive unmyelinated nerve terminals were sometimes closely attached to the pinealocytes, although no membrane specialization was noticed. SRIH-positive nerve fibers was distributed in the central parenchyma.