CDCA在C57BL/6小鼠中上调肝脏成纤维细胞生长因子21表达及其效应

目的研究法尼酯X受体(farnesoid X receptor,FXR)的激活对C57BL/6小鼠肝脏中纤维细胞生长因子21(fibroblast growth factor 21,FGF21)基因表达的影响。方法将18只3～4周龄C57BL/6小鼠随机分为3组,分别使用不同浓度鹅脱氧胆酸(chenodeoxy cholic acid,CDCA,0、10 mg/kg和50 mg/kg)给小鼠灌胃7 d,处死后分别提取小鼠肝脏总RNA和总蛋白,采用RT-PCR法和Western blot法检测各组小鼠FGF21RNA和蛋白表达水平的变化;同时用酶法测定检测小鼠血甘油三酯(TG)、胆固醇(TC)及血糖(GLU)变化。结果 C57BL/6小鼠肝脏中FGF21 RNA和蛋白的表达水平呈CDCA剂量依赖型增高;小鼠血脂及血糖测定结果显示:TG,TC和血糖随CDCA浓度的升高而下降。结论 CDCA可上调C57BL/6小鼠肝脏中FGF21的表达并导致血TG、TC及GLU下降。

Chenodesoxycholic acid up-regulates fibroblast growth factor 21 and its effect on C57BL/6 mice

Objective To determine the activation of the farnesoid X receptor(FXR) on the expression of fibroblast growth factor 21(FGF21) in C57BL/6 mice.Methods A total of 18 male C57BL/6 mice(3 to 4 weeks old) were randomly divided into 3 groups and treated with FXR agonist,chenodesoxycholic acid(CDCA) at different doses of 0,10,and 50 mg/kg respectively for a week.After the treatment,the mice was sacrificed for their liver tissue.RT-PCR and Western blotting were performed to test the expression of FGF21 at mRNA and protein levels in the liver tissue.Blood triglycerides(TG),cholesterol(TC) and blood glucose(GLU) were measured by enzymatic determination test.Results FXR agonist CDCA up-regulated the FGF21 at mRNA and protein levels in a dose-dependent manner in C57BL/6 liver tissue after treatment for a week.While,blood concentrations of TG,TC and GLU were decreased with the increase of CDCA doses.Conclusion CDCA up-regulates FGF21 expression in C57/BL6 mice liver and results in a decrease in plasma TG,TC and blood glucose.