CD109、HMW-CK、Vimentin、p63和AR在基底细胞样乳腺癌中的表达及其临床意义

目的 探讨CD109、高分子量细胞角蛋白（HMW-CK）、波形蛋白（Vimentin）、p63和雄激素受体（AR）在基底细胞样乳腺癌（BLBC）中的表达及其与临床病理特征的关系。方法 将ER、HER-2均阴性，CK5／6和／或EGFR阳性表达者定义为BLBC，采用免疫组化SP法检测CD109、HMW-CK、Vimentin、p63和AR在86例BLBC、57例管腔细胞型（Luminal）乳腺癌和33例HER-2 过表达型乳腺癌中的表达情况。结果 CD109在BLBC、Luminal型乳腺癌和HER-2 过表达型乳腺癌组织中的阳性表达率分别为61.6％，8.8％和9.1％，HMW-CK分别为90.7％、26.3％和33.3％，AR分别为31.4％、71.9％和42.4％，组间差异均有统计学意义（P＜0.05）；Vimentin的阳性表达率分别为57.0％、42.1％和51.5％，p63 分别为31.4％、29.8％和24.2％，组间差异均无统计学意义（P＞0.05）。在BLBC中CD109、HMW-CK、Vimentin和p63的表达与临床病理特征无关（P＞0.05），而AR的表达与组织学分级有关（P＜0.05）。结论 CD109和HMW CK是诊断BLBC的特异性标记物，而Vimentin和p63无诊断意义，AR表达缺失与BLBC的侵袭和恶性表型有关。

Expressions of CD109, HMW-CK, vimentin, p63 and AR in basal-like breast cancer

Objective To investigate the expressions and significance of CD109, high molecular weight cytokeratin（ HMW- CK） , vimentin, p63 and antrogen receptor（AR） in basal-like breast cancer（BLBC）. Methods ER and PR-negative and CK5/6 and/ or ECFR-postive expression was defined as BLBC. Immunohistochemical method was used to determine the expressions of CDI09, HMW-CK, vimentin, p63 and AR in 86 cases of BLBC, compared with 57 cases of luminal type and 33 cases of HER-2 over-expressed breast cancer. Results The positive rates of CD109 were 61.6% , 8. 8% and 9. 1% in BLBC, luminal type and HER-2 over-expressed breast cancer, while HMW-CK were 90.7% , 26. 3% and 33.3% , and AR were 31.4% , 71.9% and 42.4%. There was significant difference among them（ P 〈 0. 01 ）. The positive rates of vimentin was 57.0% , 42. 1% and 51.5% , and p63 were 31.4% , 29. 8% and 24. 2% respectively. The difference was not significant among them（P 〉0. 05）. The expression of CD109, HMW-CK,vimentin and p63 were not correlated to age, tumor size, histological grade and lymphadenopathy. AR was correlated to histological grade in BLBC. Conclusion CD109 and HMW-CK are the specific markers for the diagnosis of BLBC, while vimentin and p63 are not. The lack of AR expression in BLBC is related to invasion of tumor and malignant phenotype.