家蝇对爱比菌素的吸收和吸收抗性研究

<正> 表皮穿透是杀虫剂药理动力学的重要内容之一,杀虫剂表皮穿透能力下降又是抗性产生的一个重要原因,而且这种穿透作用的改变和其它抗性机制组合就会大大提高抗性水平。美国和英国都报道了家蝇的表皮穿透抗性由第Ⅲ染色体上的一个隐性基因控制,后来证明他们各自报道的这两个基因是一对等位基因、说明家蝇的许多种群对杀虫剂存

INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT Insecticide Uptake and Decreased Uptake Resistance in the House Fly(Diptera:Muscidae):A Study with Avermectin

We examined the pharmacokinetics of insecticide uptake and decreased uptake resistance to insecticides in the house fly,Musca domestica L. Flies were briefly exposed to low (2ng)or high (200ng)residues of 3H]avermectin in glass vials and then transferred to clean vials Adsorption occurred within 2 min and to a similar extent in susceptible and resistant flies. After transferring exposed flies to clean vials,much of the adsorbed avermectin was lost within 15 min and the loss was greater in resistant flies. In contrast,uptake of avermec-tininto the hemolymph was more extensive in susceptible flies. Preexposing flies to dinitro-phenol blocked avermectin uptake and loss in both strains,suggesting avermectin transport may be facilitated by an adenosine triphosphate-dependent protein. When fasted flies were fed14C]glucose followed by exposure to avermectin,transport of 14C]1abeled derivatives of glucose to the cuticular surface was decreased relative to controls in both strains. These results suggest that the natural function of a protein that facilitates insecticide uptake is to transport hvdrocarbons from the insect hemolymph to the cuticle and that quantitative or qualitative changes in the protein may relate to decreased uptake resistance.