人体小细胞肺癌细胞系LTEP—sm的细胞遗传学研究

应用G显带技术对我国第一个小细胞肺癌细胞系(SCLC)LTEP-sm进行细胞遗传学研究,共分析分散及显带良好的55代和99代中期相细胞各30个,并对55代及99代各50个中期相细胞进行染色体计数。结果表明,该细胞系55代细胞染色体众数为51～53条,99代为51～54条,基本是一亚三倍体。恒定出现的标记染色体有6个,其中3号染色体短臂缺失即del3p14-23在30个中期相中高达26(55代)及28(99代)个;另5个标记染色体是t(1;15)(1qter→1p22∷15p12→15qter),del(1)(qter→p31:),del(7)(pter→q31:),t(6;9)(6pter→6q14∷9q21→9qter),del(12)(qter→p12.3:)。99代细胞的染色体众数与55代基本一致,且标记染色体完全相同,表明该细胞系遗传结构稳定。

Cytogenetic Studies on One Human Small Cell Lung Cancer (SCLC)Cell Line, LTEP-sm

Cytogenetic studies on LTEP-sm, the first SCLC cell line established in China, were carried out with G-banding techniques. Two 30-metaphase groups showing good G-banding patterns in generation 55 and 99 respectively were subjected to karyological analysis. The results are as follows: the modal number of chromosomes is 51-53 (generation 55) and 51-54 (generation 99). The cell line is hypotriploid. Six marker chromosomes were found. The frequency of interstitial deletion of the short arm of chromosome 3 (del 3p14-23) was as high as 26 (generation 55) and 28 (generation 99) in the two 30-metaphase groups analyzed. This result is in accordance with reports on the genetic markers of SCLC and SCLC variants published abroad. Another 5 marker chromosomes are: t(1;15) (1qter----1p22::15p12----15qter); del(1) (qter----p31:); del (7)(pter----q31:); t(6;9)(6pter----6q14:: 9q21----9qter); and del (12)(qter----p12.3). The incidence of the marker chromosomes was also calculated.