胃粘膜S100^＋树突状细胞的免疫组织化学

应用S100蛋白(S100)抗体ABC免疫酶标法观察胎胃、正常及病变胃粘膜间质中的S100~ 树突状细胞(S100~ DC),其中胎胃11例、胃溃疡19例、慢性萎缩性胃炎28例、胃癌65例及正常对照15例。结果表明,正常胃和胎胃粘膜间质中仅有少量DC,而良、恶性病变的胃粘膜间质中DC数量显著增多,其分布与淋巴细胞密切相关。提示胃粘膜DC变化与抗原的种类、数量及个体免疫应答能力有关,DC在淋巴细胞激活过程中起抗原提呈作用。

Immunohistochemical Characterization of S100 Proteinpositive Dendritic Cells in Gastric Mucosa

Immunohistochemical staining was performed by the ABC method using antibody against S100 protein for the identification of dendritic cells (DC)in the gastric mucosa, including gastric ulcer (n=19), chronic atrophic gastritis (n=28), gastric carcinoma (n=65), fetal gastric mucosa (n=11)and normal controls (n=15). Lower numbers of DC may indicate an antigen-deficient state in normal and fetal gastric mucosa, while the diverse densities of DC in pathological conditions may reflect the differences in antigenic quantities and qualities and in individual immune responses. The close association between DC and lymphocytes in situ suggests an antigen-presenting capacity of DC controlling, the activation of lymphocytes.