| DMSO诱导下蛋白激酶C与HL—60细胞分化的关系 |
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| 引用本文： | 汪黎 糜静娴. DMSO诱导下蛋白激酶C与HL—60细胞分化的关系[J]. 中国医学科学院学报, 1990, 12(2): 96-100 |
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| 作者姓名： | 汪黎 糜静娴 |
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| 作者单位： | 中国医学科学院血液学研究所 天津(汪黎，糜静娴)，中国医学科学院血液学研究所 天津(范启修) |
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| 摘 要： | HL-60细胞在DMSO诱导下沿粒细胞方向分化,120h NBT~ 细胞达84％。细胞经诱导24h蛋白激酶C活性增加,72h为未诱导细胞的2.7倍。酶活性的增加,主要是由于细胞浆部分而不是细胞膜部分可溶性酶活性改变的结果。分化诱导剂DMSO本身不能直接激活蛋白激酶C,提示DMSO对酶活性的影响是间接的。
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| 关 键 词： | 蛋白激酶C HL－60细胞 二甲基亚砜 |
Correlation between Protein Kinase C and Differentiation of HL-60 Cells Induced by DMSO |
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| Wang Li,et al. Correlation between Protein Kinase C and Differentiation of HL-60 Cells Induced by DMSO[J]. Acta Academiae Medicinae Sinicae, 1990, 12(2): 96-100 |
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| Authors: | Wang Li et al |
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| Affiliation: | Institute of Hematology, Tianjin. |
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| Abstract: | Treatment of HL-60 cells with DMSO caused cells to differentiate along the myeloid way and 84% of the cells showed a reduction in NET after 120h. Protein kinase C activity increased of during differentiation. It would reach 2.7 times that of the control after 72h in the presence of DMSO. The increase of protein kinase C activity was traced to the cytosolic rather than the membrane fraction. Chelator EGTA was necessary to extract protein kinase C. DMSO itself could not enhance protein kinase C activity, indicating that the effect of DMSO was indirect. All of these results showed that the activation of protein kinase C is important in the differentiation of HL-60 cells. |
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| Keywords: | protein kinase C HL-60 cells |
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