大肠癌中VEGF、Ki-67和MVD的检测及其临床意义

目的：探讨大肠癌组织VEGF、Ki-67的表达和微血管密度（MVD）及其临床意义。方法：应用免疫组化SP法检测60例大肠癌组织中VEGF、Ki-67的表达和MVD，并分析其临床特征及相关性。结果：大肠癌VEGF、Ki-67的阳性表达率分别为76.7%、73.2%，明显高于癌旁肠组织的35.0%、20.1%。两者比较差异均有统计学意义（P〈0.05）；VEGF阳性率在有/无淋巴结转移的大肠癌中差异显著（P〈0.05）；VEGF阳性表达组MVD值显著高于阴性组（P〈0.05）。结论：VEGF与大肠癌的发生、发展和转移密切相关，提示阻断VEGF通路将对大肠癌的治疗提供新的靶点。Ki-67是肿瘤良恶性鉴别、提示预后的重要指标。

Significance of VEGF,Ki-67 and Intratumour MVD in Colorectal Carcinomas

Objective：To investigate effect of the expression of vascular endothelial growth factor（VEGF）,Ki-67 and on intratumour microvessel density（MVD）in colorectal carcinoma tissues. Method：The immunohistochemical（SP method）was used to detect the expression of VEGF, Ki-67 and MVD in 60 cases of colorectal carcinomas,analyzed the clinical pathological characteristics and correlation. Result：The positive expression rates of VEGF and Ki-67 of colorectal carcinoma were 76.7%and 73.2%,which were higher than that in adjacent non-neoplastic tissue（35.0%and 20.1%）（P〈0.05）. The expression of VEGF in colorectal carcinoma was correlated with lymph node metastasis（P〈0.05）. The expression of VEGF was correlated with MVD（P〈0.05）. Conclusion：VEGF might have important significance in the carcinogenesis,progression and metastasis of colorectal carcinoma,and VEGF might be as a potentially useful target for therapeutic intervention in colorectal carcinoma patients. Ki-67 plays an important value in differential diagnosis and prognosis of the tumour.