过山蕨中黄酮类成分的研究

 目的研究过山蕨(Camptosorus sibiricus Rupr.)中黄酮类化学成分。方法采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法、高效液相色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定了其化学结构。结果从过山蕨70%乙醇提取物中分离得到6个黄酮类化合物,并鉴定其结构为:山柰酚(1);山柰酚-7-O-α-L-鼠李糖苷(2);山柰酚-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖苷(3);山柰酚-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖基-7-O-β-D-吡喃葡萄糖苷(4);山柰酚-3-O-β-D-吡喃葡萄糖基-7-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖苷(5);山柰酚-3-O-β-D-吡喃葡萄糖基(1→4)-α-L-鼠李糖基-7-O-α-L-鼠李糖苷(6)。结论化合物2,3,4,5,6均为首次从该属植物中分离得到。

Studies on Flavonoidal Constituents from Camptosorus sibiricus Rupr.

OBJECTIVE To study the flavonoidal constituents from the 70% ethanol extract of the whole herbal of Camptosorus sibiricus Rupr..METHODS The compounds were isolated by chromatography on silica gel column,Sephadex LH-20 column,preparative HPLC column,and identified on the basis of physico- chemical constants and spectral analysis. RESULTS Six compounds were isolated as kaempferol (1),kaempferol-7-O-α-L-rhamnoside (2),kaempferol 3-O-β-D-glucopyranosyl (1→2) -β-D- glucopyranoside (3),kaempferol- 3-O-β-D- glucopyranosyl (1→2) -β-D-glucopyranosyl -7- O-β-D- glucopyranoside (4),kaempferol-3-O-β-D- glucopyranosyl-7-O-β-D- glucopyranosyl-(1→2)-β-D-glucopyranoside (5),kaempferol 3-O-β-D-glucopyranosyl (1→4) -α-L- rhamnosyl -7-O-α-L-rhamnoside (6) ,respectively.CONCLUSION Compounds 2,3,4,5,6 are obtained from this genus for the first time.