| 超临界CO2萃取白芷中欧前胡素的研究 |
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| 引用本文： | 刘文,韩笑,王学勇.超临界CO2萃取白芷中欧前胡素的研究[J].中国药学杂志,2003,38(2):99-100. |
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| 作者姓名： | 刘文 韩笑 王学勇 |
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| 作者单位： | 贵阳中医学院,贵州,贵阳,550002 |
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| 基金项目： | 贵州省卫生厅 2 0 0 0年优秀青年科技人才基金课题 |
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| 摘 要： | 目的研究超临界流体CO2萃取白芷中欧前胡素的提取工艺，并用高效液相色谱法测定欧前胡素的含量。方法在Inertsil ODS-3柱上，以甲醇-水(65∶35)为流动相，流速:1.5 mL·min-1，检测波长：248 nm，柱温:40℃。结果欧前胡素浓度在0.0415～0.166 mg·mL-1范围内呈线性关系(r=0.999 8);加样平均回收率：99.12%，RSD为2.73%。结论采用SFE萃取白芷中欧前胡素的提取工艺速度快,HPLC测定欧前胡素的含量精确、重现性好。
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| 关 键 词： | 超临界CO2流体萃取法 白芷 高效液相色谱法 欧前胡素 |
| 文章编号： | 1001-2494(2003)02-0099-02 |
| 收稿时间： | 2002-03-13; |
Study on extraction of imperatorin in Angelica dahurica by supercritical fluid |
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| LIU Wen,HAN Xiao,WANG Xue yong.Study on extraction of imperatorin in Angelica dahurica by supercritical fluid[J].Chinese Pharmaceutical Journal,2003,38(2):99-100. |
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| Authors: | LIU Wen HAN Xiao WANG Xue yong |
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| Institution: | Guiyang College of Traditional Chinese Medicine,GuiYang 550002,China |
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| Abstract: | OBJECTIVE To investigate the extraction technology of imperatorin in Angelica dahurica by supercritical fluid,and to determine the content of imperatorin by HPLC.METHOD S The inertsil ODS 3 column was used with a mobile phase of methyl alcohol water(65∶35).The flow rate was 1.5 mL·min-1.The detection wavelength was at 248 nm.The column temperature was 40℃.RESULTS The calibration curve of imperatorin was linear over the range of 0.041 5～0.166 mg·mL-1(r=0.999 8).The average recoveries were 99.12% with RSD 2.73%.CONCLUSION The extraction technology was rapid,and the HPLC assay was accurate and reproducible. |
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| Keywords: | CO 2 SFE Angelica dahurica HPLC imperatorin |
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