原癌基因 c- kit mRNA在自身免疫性睾丸炎中的表达及意义

 目的]探讨原癌基因c-kit mRNA在自身免疫性睾丸炎中的表达及意义.方法]建立并分析自身免疫性睾丸炎动物模型：42只BALB/c小鼠随机分为6个实验组和1个对照组.6个实验组小鼠分别给予KM小鼠睾丸混悬液皮下注射,每周1次最多者4次,在第1次给予抗原注射后1,2,3,4,6,8周各取一组小鼠的睾丸组织,用RT-PCR检测其c-kit mRNA的变化.结果]在第1次给予抗原注射后第1周开始c-kitm RNA转录水平开始降低,第2周起明显低于对照组（P＜0.05）,至第3,4周降至最低,第6周逐渐开始恢复,至第8周其水平与对照组相比差异已经没有统计学意义（P＞0.05）.结论]c-kit基因与生精功能密切相关,c-kit基因mRNA的低表达可能参与自身免疫性睾丸炎导致不育的发病机制.

Expression and Role of Oncogene c-kit mRNA in Development of Experimental Autoimmune Orchitis

Objective] To investigate the expression of oncogene c-kit mRNA and its role in the development of experimental autoimmune orchitis. Methods] The models of experimental autoimmune orchitis were established and analyzed: 42 BALB/c mice were divided into 6 experimental groups and 1 control group randomly. The testis homogenates of KM mice were respectively infected subcutaneously in 6 experimental groups every week, 4 times at most. From the first injection on, the testises were excised at 1st, 2nd , 3rd , 4th, 6th, and 8th week, each time 1 group were excised. The expression of c-kit mRNA was examined in the mice by RT-PCR. Results] The expression of c-kit mRNA was decreased after the 1st week of first injection with the control group, and from the 2nd week on, the difference was significant (P<0.05) till the 3rd and 4th week it reached to its lowest level. From the 6th week on, it began to come back, and at the 8th week the difference was no significant (P >0.05) with the control group.Conclusion] Oncogene c-kit is related with spermatogonial. The down-regulation of c-kit mRNA in experimental autoimmune orchitis might participate in the pathogenesis of infertility.