Inhibition of Intercellular Communication in Cultures of Chinese Hamster V79 Cells by 2,4-Dichlorophenoxyacetic Acid and 2,4,5-Trichlorophenoxyacetic Acid

Inhibition of Intercellular Communication in Cultures of ChineseHamster V79 Cells by 2,4-Dicfalorophenoxyacctic Acid and 2,4,5-TrichlorophenoxyaceticAcid. RUBINSTEIN, C, JONE, C, TROSKO, J. E., AND CHANG, C. C.(1984). Fundam. Appt. Toxicol. 4, 731–739. Using the Chinesehamster V79 in vitro cell system designed to measure intercellularcommunication, 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyaceticacid (2,4,5-T), and several mixtures of these compounds weretested for their ability to inhibit this biological process.The ability of these chemicals to inhibit colony-forming abilityof these cells was tested prior to the studies to measure intercellularcommunication. 2,4-D was less cytotoxic than 2,4,5-T. Both 2,4,5-Tand 2,4-D were able to inhibit intercellular communication attheir respective noncytotoxic dose ranges. Various mixturesof both chemicals were also able to inhibit intercellular communication,snowing some kind of addititvity. No-effect levels were alsonoted in the intercellular communication assay. These resultswere interpreted as being consistent with the hypothesis thatthese compounds might be teratogenic by their ability to inhibitintercellular communication during development