人类白细胞抗原G和E在人早孕胎盘组织中的表达及其与反复自然流产的关系

目的 探讨人类白细胞抗原(HLA)-G、E在人早孕胎盘组织中的表达分布及其与反复自然流产(RSA)发生的关系.方法 选择15例正常早孕妇女及15例RSA患者为研究对象.分别采用原位杂交方法及免疫组织化学法检测HLA-G、E的mRNA和蛋白质在早孕胎盘组织中的表达.结果 在人早孕胎盘组织所有的滋养细胞,包括合体滋养细胞、绒毛细胞滋养细胞、绒毛外滋养细胞中均有HLA-G、E mRNA的表达.特异性针对HLA-G的单克隆抗体4H84仅在绒毛外滋养细胞中检测出HLA-G蛋白表达.HLA-E蛋白表达与其mRNA表达部位一致.RSA组-眦A-G、EmRNA的表达强度明显低于正常对照组.HLA-G、E蛋白的表达水平在RSA组也明显降低.结论 HLA-G mRNA与蛋白表达部位的不一致可能与单克隆抗体4H84有关.母胎界面的滋养细胞表面HLA-G、E mRNA及蛋白表达水平的下降与RSA的发生密切相关.

The Expression of Human Leukocyte Antigen G and E on Human First Trimester Placenta and Its Relationship with Recurrent Spontaneous Abortion

Objective To investigate the distribution of human leukocyte antigen(HLA)-G and E on human first trimester placenta and its relationship with unexplained recurrent spontaneous abortion(RSA).Methods Fifteen women with normal first trimester pregnancy and fifteen patients with RSA were included in this study.In situ hybridization and immunohistochemical staining were employed to detect the mRNA and protein of HLA-G,E in first trimester placenta.Results The HLA-G,E mRNA were detected in all trophoblasts,including syncytiotrophoblast,villous and extravillous cytotrophoblast.HLA-G protein was only found at extravillous trophoblast by mAb 4H84 specific to HLA-G.The same expression sites were observed for both HLA-E protein and its mRNA.The expression of HLA-G,E mRNA in the RSA group were much lower than those in the control group.The HLA-G,E protein were also expressed at low level in RSA group.Conclusion The inconsistency of the distribution of HLA-G mRNA and protein was probably due to the monoclone antibody 4H84.There is strong correlation between lack of the mRNA and protein expression of HLA-G,E found in the trophoblasts at the fetal-maternal interface and RSA.