尿胰蛋白酶抑制剂Ulinastatin 对lewis 肺癌小鼠肿瘤生长和转移的抑制作用

 目的　探讨尿胰蛋白酶抑制剂(U TI) 在动物模型上抗肿瘤作用。方法　选用尿胰蛋白酶抑制剂乌司他丁(Ulinastatin) , Lewis 肺癌小鼠模型;雄性C57BL/ 6 小鼠40 只,接种lewis 肿瘤细胞,分成生理盐水组(对照组) 、环磷酰胺组(CTX) 、Ulinastatin 2. 5万单位组、Ulinastatin 5 万单位组、Ulinastatin 10万单位组,各8 只,接种后第6 天,开始腹腔给药,记录皮下瘤长短径变化,做生长曲线;接种14 天后处死所有小鼠,统计皮下平均瘤重和肺转移灶个数, 使用流式细胞计分析凋亡率(AR) 增值百分比(SPF) 。结果　皮下瘤重依次为(7. 92 ±2. 52 、0. 66 ±0. 50^＊＊ 、3. 47 ±1. 45^＊ 、3. 08 ±0. 81^＊＊、1. 70 ±1. 05^＊＊) g ,平均肺转移灶依次为( 8. 625 ±1. 407 、1. 125 ±1. 126^＊＊、1. 625 ±1. 302^＊＊ 、1. 00 ±0. 75^＊＊ 、0. 625 ±0. 74^＊＊) 。CTX 组(39. 3 ±4. 8) %^＊ 和Ulinastatin 10 万单位组(40. 2 ±3. 1) %^＊的AR 值明显增加,Ulinastatin 未见SPF 值明显减少。结论　Ulinastatin 对lewis 肺癌小鼠的转移瘤生长和自发性肺转移有抑制作用。

Inhibition of Metastasis and Invasion of Lewis Lung Carcinoma by Urinary Trypsin Inhibitor

Objective 　To investigate whether urinary t ryp sin inhibitor inhibit s tumor invasion and metastasis of lewis lung carcinoma mice . Methods 　Subcutaneous ( s. c. ) implantation of 3LL cells (5. 0 ×10⁶ ) in the right subcutaneous armpit of C57BL/ 6 male mice. There were forty mice divided into five groups with random. There were physiological saline group , cyclophosphamide group , U TI2. 5 ×10⁴ u 7d group ,U TI 5. 0 ×10⁴ u 7d group ,U TI 10. 0 ×10⁴ u 7d group. They all started to inject at sixth day by abdominal cavity . The volume of tumors were measured at the 8th day ,10 th day ,12th day . The weight of primary tumor and lung metastasis were established by the 14 th day af ter tumor cell inoculation . Flow Cytometry was used to analyze apoptosis rate and s-phase f raction. Results 　The average weight of tumor in turn were (7. 92 ±2. 52 、0. 66 ±0. 50 ^＊＊、3. 47 ±1. 45^＊3 、3. 08 ±0. 81^＊＊3 3 、1. 70 ±1. 05^＊＊ ) g , the average number of lung metastasis were (8. 625 ±1. 407 、1. 125 ±1. 126^＊＊ 、1. 625 ±1. 302^＊＊ 、1. 00 ±0. 75^＊＊ 、0. 625 ±0. 74^＊＊) . The apoptosis rates of CTX (39. 3 ±4. 8) %^＊and U TI 10. 0 ×104 u ( 40. 2 ±3. 1) %^＊ weremarkedly increased. The s2phase f ractions of U TI cannot reduce s-phase f raction. The tumor growthcurve showed in FIG. 3. Conclusion 　Ulinastatin can inhibit primary tumors and lung metastasis carcinoma of Lewis mice.