| 睾丸组织异体异位移植生成成熟精子的动物模型建立 |
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| 引用本文： | 于洁,叶静,张芳婷,万汇涓,尹美珺,房家智,桂耀庭,蔡志明.睾丸组织异体异位移植生成成熟精子的动物模型建立[J].北京大学学报(医学版),2004,36(6):591-594. |
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| 作者姓名： | 于洁 叶静 张芳婷 万汇涓 尹美珺 房家智 桂耀庭 蔡志明 |
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| 作者单位： | 北京大学深圳医院生殖医学中心男性生殖实验室,深圳,518036;北京大学深圳医院中心实验室,深圳,518036 |
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| 基金项目： | 广东省自然科学基金
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广东省深圳市科技计划 |
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| 摘 要： |
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| 关 键 词： | 睾丸 组织移植 精母细胞 睾丸特异蛋白激酶 小鼠 |
| 文章编号： | 1671-167X(2004)06-0591-04 |
| 修稿时间： | 2004年6月30日 |
Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis |
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| YU Jie.Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis[J].Journal of Peking University:Health Sciences,2004,36(6):591-594. |
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| Authors: | YU Jie |
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| Institution: | Laboratory of Male Reproduction, Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China. yujie007@hotmail.com |
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| Abstract: | Objective:To establish an in vitro model for the development of mouse spermatogenic cellsinto sperm by using the immunodefective mouse as the incubator.Methods:Tissue grafting was per-formed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipi-ents;the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis fur-ther observed with histological analysis of grafts.Results:Molecular biological and histological analysesshowed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis,but also ex-hibited similarities in the structure of seminiferous tubules and component of spermatogenic cells,inclu-ding sperms.Conclusion:Spermatogenic cells heterotopically grafted in vitro could continuously growand complete spermatogenesis and finally develop into sperm. |
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| Keywords: | Testis Tissue transplantation Spermatocytes Testis-specific protein kinase 1 Mice |
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