| 鼻咽癌低分化细胞系CNE-2双向凝胶电泳图谱的建立 |
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| 引用本文： | 杨海燕,肖志强,李峰,余艳辉,张鹏飞,陈主初.鼻咽癌低分化细胞系CNE-2双向凝胶电泳图谱的建立[J].中南大学学报(医学版),2004,29(2):123-128. |
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| 作者姓名： | 杨海燕 肖志强 李峰 余艳辉 张鹏飞 陈主初 |
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| 作者单位： | 中南大学,湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008;中南大学,湘雅医学院肿瘤研究所,长沙,410078;中南大学,湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008;中南大学,湘雅医学院肿瘤研究所,长沙,410078 |
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| 基金项目： | 国家“973”计划 (2 0 0 1CB51 0 2 )，国家自然科学基金 (30 0 0 0 0 2 8，30 2 4 0 0 56，30 370 642 )，教育部跨世
纪优秀人才培养计划基金 (教技函 [2 0 0 2 ] 4 8)，湖南省科技厅重点科研项目 (0 2 5SY2 0 0 1 1 )，湖南省卫生厅重点科研项目 (Z0 2 4) |
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| 摘 要： | 目的：建立人鼻咽癌低分化细胞系CNE-2双向凝胶电泳图谱，初步分析其蛋白质表达情况。方法：采用双向凝胶电泳（2-DE）分离人鼻咽癌低分化细胞系CNE-2总蛋白，银染显色，Image Master图象分析系统分析，从胶中选取分离较好的蛋白质点，基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）分析获取肽质量指纹图谱（PMF），Mascot软件搜索MSDB和SWISS-PROT数据库鉴定蛋白质。结果：获得重复性和分辨率较好的CNE-2双向凝胶电泳图谱；质谱分析了78个蛋白质点，获取77张肽质量指纹图谱，通过查询数据库鉴定了48个蛋白质。结论：建立了人鼻咽癌低分化细胞系CNE-2双向凝胶电泳图谱，并应用质谱技术鉴定了部分蛋白质点，为人鼻咽癌蛋白质组数据库的建立提供了有意义的数据。
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| 关 键 词： | 双向凝胶电泳 鼻咽癌 蛋白质组 |
| 文章编号： | 1672-7347(2004)02-0123-06 |
| 收稿时间： | 2003-12-17 |
| 修稿时间： | 2003年12月17 |
Establishment of 2-DE map of human low differentiation
nasopharyngeal carcinoma cell line CNE-2 proteome |
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| YANG Hai yan ,XIAO Zhi qiang ,LI Feng ,et al..Establishment of 2-DE map of human low differentiation
nasopharyngeal carcinoma cell line CNE-2 proteome[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2004,29(2):123-128. |
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| Authors: | YANG Hai yan XIAO Zhi qiang LI Feng |
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| Institution: | 1.Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital, Central South University, Changsha 410008；
2. Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China |
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| Abstract: | Objective To establish a two dimensional polyacrylamide gel electrophoresis(2 DE) map of human low differentiation nasopharyngeal carcinoma (NPC) cell line CNE 2 proteome. Methods Immobilized pH gradient 2 DE was applied to separate the total proteins of CNE 2 cells, matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) and database searching were used to indentify proteins. Results A good 2 DE pattern with high resolution and reproducibility was obtained. Seventy eight protein spots were incised from sliver staining gel and digested in gel by trypisin. Of them 77 maps of peptide mass fingerprints (PMF) were obtained and 48 proteins were identified.Conclusion A reference 2 DE map of CNE 2 proteome has been established and the data coupled with similar proteome analysis of other NPC cell lines will expand the human nasopharyngeal carcinoma proteome database. |
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| Keywords: | two dimensional polyacrylamide gel electrophoresis human nasopharyngeal carcinoma proteome |
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