创伤性脑损伤后尼莫同对AQP4表达和血脑屏障通透性的影响

目的:研究脑损伤(TBI)后尼莫同对AQP4表达和血脑屏障(BBB)通透性的影响。方法:健康成年Wistar大鼠,自由落体硬膜外撞击方法致重度脑创伤模型,随机分成尼莫同组和生理盐水对照组。每组观察点定为伤后4h、8h、12h、1d、3d、5d和7d。取大鼠脑组织进行以下实验:(1)HE染色进行形态学观察;(2)免疫组化检测AQP4的表达变化;(3)测伤区脑组织中EB外渗的量,反映BBB通透性的变化。结果:脑损伤后,在尼莫同组和对照组缺血组织均出现脑损伤的病理改变、AQP4表达上调、BBB通透性增加,其变化趋势一致;且随伤后时间延长改变加重。除AQP4表达外,其他各项指标的差别从TBI12h开始具有统计学意义(P<0.05)。结论:脑损伤后,尼莫同可以增加BBB通透性,加重脑损伤,不影响AQP4的表达。

Effect of Nimodipine on expression of AQP4 and permeability of BBB after traumatic brain injury

Objective:To explore effect of Nimodipine on expression of AQP4 and permeability of blood brain barrier(BBB)after traumatic brain injury.Methods:Made severe traumatic brain injury mode of Wistar rat with epidural impact by free falling.Randomly separated all rats into Nimodipine group and saline control group.Observed rats at 4h,8h,12h,1d,3d,5d and 7d after injury respectively.Took cerebral tissue for following experiments:(1)Morphological observation after HE stain;(2)Immunohistological experiment for testing expression of AQP4;(3)Determination of EB in cerebral injury tissue to observe the permeability of BBB.Results:After brain injury,pathological variation,up-regulation of AQP4 expression,increase of BBB permeability occurred in ischemic tissue both of Nimodipine group and control group.Besides,the longer the injury lasted,the more severe these pathological variations were.Except expression of AQP4,differences of all indexes were significant between two groups from 12h(P<0.05).Conclusion:Nimodipine can increase permeability of BBB,and has no effect on expression of AQP4.