补骨脂素对人类乳腺癌细胞增殖作用的影响

目的研究补骨脂素(psoralen,Pso)对人类乳腺癌细胞株增殖的影响。方法采用噻唑蓝(MTT)比色法测定补骨脂素对雌激素依赖性乳腺癌细胞MCF-7和非雌激素依赖性乳腺癌细胞MDA-MB231的细胞增殖作用,并以雌激素受体拮抗剂ICI182,780为工具药来评价补骨脂素发挥雌激素样作用与雌激素受体的关系,流式细胞术对MCF-7细胞的增殖情况进行分析。结果与溶剂对照组相比较,Pso(10～40μmol.L-1)能明显促进MCF-7细胞的增殖,而对雌激素受体阴性MDA-MB231细胞未见影响,并将MCF-7细胞周期由G1期向S期推进,促进DNA合成,提高细胞分裂增殖指数,且Pso促进MCF-7细胞增殖作用被雌激素受体拮抗剂所拮抗。结论补骨脂素具有雌激素活性,此作用是通过雌激素受体(ER)介导的。

Effect of psoralen on proliferation of human breast carcinoma cells

Aim To investigate the effects of psoralen on the proliferation of human breast carcinoma cells in vitro.Methods Effects of psoralen on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231cell by MTT assay.The estrogen-like effect of psoralen and its relation with the estrogen-receptor were evaluated by using pure estrogen receptor antagonist ICI182,780 as a tool.Proliferation of MCF-7 cell was examined by flow cytometer.Results Psoralen(10～40 μmol·L-1)stimulated proliferation of ER-positive MCF-7 cell compared with solvent control,whereas effect on ER-negative MDA-MB231 cell proliferation was not found,the cell cycle was impulsed from G1 to S,DNA synthesizing was enhanced,and PI also increased.The above function of boosting MCF-7 cell proliferation could be inhibited by adding estrogen receptor antagonist ICI182,780.Conclusions Psoralen has the estrogen-like activities through the estrogen response pathway.