乌司他丁对严重烧伤大鼠延迟复苏的影响

目的:探讨乌司他丁对严重烧伤延迟复苏的作用。方法:18只SD大鼠随机分成假烫组(S组)、烫伤延迟复苏组(B组)和烫伤延迟复苏+乌司他丁组(U组)。U组在烫伤后延迟复苏的同时,于伤后6h、12h分别予乌司他丁20 000 U·kg~(-1)腹腔内注射。伤后24h处死大鼠,测定伤后24h肺、肝干湿重比,测定血清白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)、乳酸值和血浆丙二醛含量。结果:B组IL-8、TNF-α、乳酸和丙二醛含量均高于S组,肺、肝干湿重比低于S组(P<0.01),U组大鼠肺、肝干湿重比为0.229 4±s 0.001 2、0.275±0.008,血清IL-8和TNF-α值为(226±16)ng·L~(-1)、(514±24)ng·L~(-1),血清乳酸与血浆丙二醛含量分别为(4.5±0.8)mmol·L~(-1)、(2.52±0.27)nmol·L~(-1),均比B组有明显改善(P<0.05或P<0.01)。结论:乌司他丁可减轻严重烧伤延迟复苏对机体的损伤。

Effects of ulinastatin on delayed resuscitation of severely burned rats

AIM:To explore the effects of ulinastatin on delayed resuscitation of severe burns.METHODS: Eighteen SD rats were randomly divided into 3 groups:sham group (S group),burned and delayed resuscitation group (B group) and therapeutic group (U group).In U group,ulinastatin (20 000 U·kg~(-1)) was intra- peritoneally injected at 6 h,12 h after burn with simultaneously prolonged resuscitation.Rats were executed post-buru 24 h and followed by measurement of the ratios for dry weight and wet weight tissues of lungs and livers;together with calculation of the serum contents of interleukin 8 (IL-8),tumor necrosis factor-alpha (TNF-α),malondialdehyde (MDA) and lactic acid (LD).RESULTS:The ratios of dry and wet weight of lung and liver tissues in B group were lower than those in S group,while the levels of IL-8,TNF-α,MDA and LD were higher (P<0.01),respectively.The ratios of dry and wet weight of lung and liver tissues in U group were 0.229 4±s 0.001 2 and 0.275±0.008,while levels of IL-8,TNF-α,LD and MDA were (226±16) ng·L~(-1), (514±24) ng·L~(-1),(4.5±0.8) mmol·L~(-1) and (2.52±0.27) nmol·L~(-1);all improved much more than those in B group (P<0.05 or P<0.01 ).CONCLUSION:Ulinastatin can decrease the injury with delayed resuscitation in severe burns.