| 镁黄长石浸提液影响人脂肪干细胞增殖和成骨分化的实验研究 |
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| 引用本文： | 谷辉杰,巩伦理,张昀,尹烁,常江,崔磊. 镁黄长石浸提液影响人脂肪干细胞增殖和成骨分化的实验研究[J]. 组织工程与重建外科, 2010, 6(6): 301-305. DOI: 10.3969/j.issn.1673-0364.2010.06.001 |
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| 作者姓名： | 谷辉杰 巩伦理 张昀 尹烁 常江 崔磊 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院整复外科；上海市组织工程国家工程研究中心；上海市中国科学院上海硅酸盐研究所 |
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| 基金项目： | 国家自然科学基金(30672190和30730034) |
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| 摘 要： | 目的通过探讨不同浓度镁黄长石浸提液对人脂肪干细胞(Human adipose-derived stem cells,hADSCs)增殖和成骨分化的影响,初步阐明镁黄长石体外促进hADSCs成骨分化的机制。方法依照ISO/EN 10993-5标准,制备镁黄长石浸提液,将获得的hADSCs培养于不同浓度的浸提液中(1/2、1/4、1/8、1/16、1/32),利用MTT法检测细胞的增殖情况;在成骨诱导条件下,通过碱性磷酸酶染色、活性检测和茜素红染色以及钙离子定量检测,观察不同浓度浸提液对hADSCs成骨特性的影响。结果 1/2、1/4、1/8浓度的浸提液可以浓度依赖性地抑制hADSCs的体外增殖;1/4、1/8、1/16浓度的浸提液可以促进hADSCs的体外成骨分化;碱性磷酸酶染色和活性检测、茜素红染色和钙离子定量检测显示,1/4浓度的浸提液对hADSCs的体外成骨分化促进作用最强,此时的钙、镁和硅离子浓度分别为:2.36 mM、1.11 mM和1.03 mM。结论镁黄长石浸提液中的离子在适当浓度时,可以抑制hADSCs的体外增殖,同时促进hADSCs的体外成骨分化。
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| 关 键 词： | 人脂肪干细胞 增殖 成骨分化 镁黄长石 浸提液 |
Experimental Study on the Effect of Extracts of Akermanite on Proliferation and Osteo-Differentiation of Human Adipose-Derived Stem Cells |
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| GU Huijie,GONG Lunli,ZHANG Yun,YIN Shuo,CHANG Jiang,CUI Lei. Experimental Study on the Effect of Extracts of Akermanite on Proliferation and Osteo-Differentiation of Human Adipose-Derived Stem Cells[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2010, 6(6): 301-305. DOI: 10.3969/j.issn.1673-0364.2010.06.001 |
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| Authors: | GU Huijie GONG Lunli ZHANG Yun YIN Shuo CHANG Jiang CUI Lei |
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| Affiliation: | 1 Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai 200011,China;2 National Tissue Engineering Research and Development Center,Shanghai 200241,China;3 Shanghai Institute of Ceramics,Chinese Academy of Science,Shanghai 200050,China |
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| Abstract: | Objective To elucidate the underlying mechanism of the osteo-differentiation of akermanite on human adipose-derived stem cells(hADSCs) via observing the effect of different diluted rate extracts on the proliferation and osteo-differentiation of hDASCs.Methods The original extract was prepared according to the ISO/EN 10993-5 and hDASCs were cultured in the different diluted rate extracts(1/2,1/4,1/8,1/16,1/32).The MTT assay was carried out to quantitatively evaluate the proliferation of the cells.Under the c... |
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| Keywords: | Human adipose-derived stem cells(hADSCs) Proliferation Osteogenic differentiation Akermanite Extract |
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