高效毛细管电泳法测定四维灵芝液中核苷类成分的含量

目的 :建立毛细管电泳测定液体制剂———四维灵芝液中核苷类成分的方法。方法 :熔融石英毛细管柱 75 μmid× 5 7cm (有效长度 5 0cm) ;缓冲体系 2 0mmol·L^-1硼砂含 30mmol·L^-1SDS ,添加 5%乙醇 (95%) ,pH10 .0 (1mol·L^-1NaOH调节 ) ;气动进样时间 15s;分离电压 10kV(恒压方式 ) ;柱温 20℃ ;检测波长 254nm。结果 :回归方程腺苷 :Y=0.0705+0.01707X(r=0.9995 ) ;鸟苷:Y =0 .02 32 +0.01864X(r=0.9999)。平均回收率(n=5 ) :腺苷为 99.22% ,RSD =3.66% ;鸟苷为 104.3% ,RSD =1.91%。根据建立的方法 ,测定了9批样品腺苷与鸟苷的含量。结论 :方法简便、快速、准确、重现性好 ,可用于样品核苷类成分测定。

Determination of Nucleosides in Siweilingzhi Mixture by HPCE

OBJECTIVE: To establish a method for determining nucleosides (adenoside and guanoside) in Siweilingzhi Mixture by HPCE. METHOD: Adenoside and guanoside were separated within 25 min using an 20 mmol.L-1 borate buffer with 30 mmol.L-1 SDS and 5% Ethanol (adjusted to pH 10.0 with sodium hydroxide solution), with an operation voltage of 10 kV, temperature of 20 degrees C and a hydrodynamic injection time of 15 s. Seperations were carried out in a fused-silica capillary 75 microns id x 57 cm (effective length 50 cm) with peak detection by direct UV at 254 nm. RESULT: Regression equation of adenoside and that of guanoside were Y = 0.0705 + 0.01707X (r = 0.9995) and Y = 0.0232 + 0.01864X (r = 0.9999) respectively. The average recovery rate was 99.22% (RSD = 3.66%) and 104.3% (RSD = 1.91%) respectively. Nine samples were determined with the method. CONCLUSION: The method is simple, rapid and accurate with good repeatability and it can be used to determine nucleosides.