HPLC测定人血清中异丙酚浓度

 目的：建立反相高效液相色谱 紫外检测测定血清中异丙酚浓度的方法，并观察病人手术血清中异丙酚浓度与意识水平的关系。方法：用乙腈 高氯酸（2∶1）沉淀血清蛋白后取上清液，以邻苯二甲酸二丁酯作为内标定量。结果：本法测定的线性范围为0．5μg·ml^－1～4．0μg·ml^－1，最低检测限为0．1μg·ml^－1。血药浓度的测定说明：异丙酚无蓄积作用，维持意识消失的血药浓度为2．1μg·ml^－1以上，术后苏醒时的血药浓度约为1．0μg·ml^－1。结论：本法简单、快速，适用于手术过程中异丙酚药动学的监测。

Determination of serum propofol using high performance liquid chromatography

OBJECTIVE: To develop a method for the determination of serum propofol by using a reverse-phase HPLC and UV detector system, and to study the relationship between serum propofol concentration and unconsciousness in patients during surgical operation. METHODS: Propofol was extracted from serum by acetonitrile/perchloric acid (2∶1) precipitation and centrifugation. Dibutyl phthalate was used as the internal standard. RESULTS: The linear range and the lower limit for propofol quantification were within 0.5μg·ml^-1～4.0 μg·ml^-1 and above 0.10 μg·ml^-1, respectively. Our study suggests that the serum propofol concentration appropriate to the maintenance of unconsciousness should be more than 2.10 μg·ml^-1, and the concentration at which the patients can be awakened is approximately 1.0 μg·ml^-1. Our results also demonstrate that propofol does not accumulate in the body although it is administered continuously. CONCLUSION: The HPLC method for the measurement of serum propofol is simple and quick. It may be useful for the pharmacokinetic study during surgical operation.