灭菌丹的致突变研究

背景与目的:研究灭菌丹在不同遗传终点的致突变作用。材料与方法:采用Comet试验、Ames试验、微核试验和小鼠精母细胞染色体畸变试验分析灭菌丹在不同剂量下对DNA和染色体的损伤。结果:Comet体内试验PMNC尾长呈现出随剂量增加而增长的趋势,体外试验尾长与溶剂对照组相比,差异具有统计学意义(P<0.01)。Ames试验加和不加S9,各菌株回变菌落数均超过溶剂对照组的2倍,并呈现出剂量反应关系。微核试验和小鼠中期I精母细胞染色体畸变试验显示各剂量组与阴性对照组间差异无统计学意义(P>0.05)。结论:灭菌丹可引起鼠伤寒沙门氏菌碱基置换和移码突变,并可能损伤人外周血淋巴细胞DNA的完整性。

Mutagenic Research on Folpet

BACKGROUD & AIM: To assess the mutagenic effects of folpet at chromosomal level. MATERIALS AND METHODS: Comet assay, Ames test, micronucleus test and spermatogonial chromosomal aberration test were performed to analyze the damage of different concentrations of folpet to DNA. RESULTS: Comet test revealed that there was dose-effect relationship on rats peripheral mononuclear cells (PMNC) in vivo and there was significant difference between experiment group and negative control group on human peripheral mononuclear cells in vitro. In Ames test, there was a concentration-related increase over the range tested and a two-fold greater increase in the number of revertant colonies in four strains with or without metabolic activation. No mutagenic effect was seen in micronucleus test and spermatogonial chromosomal aberration test. CONCLUSION: Base pair substitution mutation and frame shift mutation were caused by folpet in Ames test. And DNA breakage in human PMNC might be induced by folpet.