| HPLC法测定气血双补丸中芍药苷的含量 |
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| 引用本文： | 金芳. HPLC法测定气血双补丸中芍药苷的含量[J]. 齐鲁药事, 2012, 31(2): 82-83 |
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| 作者姓名： | 金芳 |
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| 作者单位： | 淄博市药品检验所,山东,淄博,255086 |
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| 摘 要： | 目的 通过高效液相色谱法测定气血双补丸中芍药苷的含量.方法 采用高效液相色谱法,使用Agilent Zorbax SB-C18(4.6 mm×250 mm,5 μm )色谱柱,以乙腈-0.1%磷酸(14∶ 86)为流动相,检测波长为 230 nm,流速:1.0 mL·min-1,柱温:35 ℃.结果 芍药苷在0.081 28～2.032 0 μg范围内呈良好的线性关系,回归方程为Y=1 275 158X-18 633,r=0.999 9,芍药苷平均回收率为100.03%,RSD＝0.83%.结论 本法灵敏、准确、专属性强,重现性好,可用于测定气血双补丸中芍药苷的含量.
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| 关 键 词： | 气血双补丸 芍药苷 高效液相色谱法 |
Determination of paeoniflorin in Qixue Shuangbu Pill by HPLC |
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| JIN Fang. Determination of paeoniflorin in Qixue Shuangbu Pill by HPLC[J]. qilu pharmaceutical affairs, 2012, 31(2): 82-83 |
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| Authors: | JIN Fang |
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| Affiliation: | J1N Fang ( Zibo Institute .for Drug Control, Zibo 255086, China ) |
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| Abstract: | Objective To determine the content of paeoniflorin in Qixue Shuangbu Pill by HPLC. Methods HPLC was per- formed on Agilent Zorbax SB- ClS column（4.6 mm x 250 mm,5 p.m）. The mobile phase was aeetonitrile -0.1% phosphoric acid solution（ 14： 86） ,the detection wavelength was 230 nm,the flow rate was 1.0 mL · min^-l and the column temperature was 35 ℃. Results The paeoniflorin showed a good linear relationship within the range of 0.081 28 - 2. 032 0 μg, the regression e- quation was Y = 1 275 158X- 18 633 ,r = 0. 999 9 and the average recovery was 100.03% （RSD = 0. 83% ）. Conclusion This method was sensitive, accurate with good specificity and reproducibility, and it can be used for the determination of paeoniflorin in Qixue Shuangbu Pill. |
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| Keywords: | Qixue Shuangbu Pill Paeoniflorin HPLC |
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