HPLC测定天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量

目的：建立天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量测定方法。方法：采用InertsilODS（250ram×4．6mm，5斗m）色谱柱，流动相乙腈（A）一0．2％磷酸水（B）进行梯度洗脱（0—20min，A：B=2：98；20—22min，A：B=2：98一A：B：11：89），流速为1．2mL／min，检测波长220nm，柱温30~C。结果：测定天麻素在19．3—193．21xg线性关系良好（r：O．9997），松脂醇二葡萄糖苷在6．12—61．2斗g线性关系良好（r=O．9999），平均回收率分别为100．2％和98．5％。结论：本方法简便、准确，可同时测定天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量。

Determination of gastrodia element and turpentine alcohot-biglucosid in Chinese medicine compound formulations Gastrodia and Uncaria Drink by HPLC

Objective： To establish an HPLC method for determination of gastrodia element and turpentine alcohot - biglucosid in Chinese medicine compound formulations Gastrodia and Uncaria Drink. Method： The chromatographic condition was as follows： In- ertsil ODS （250mm x 4. 6mm, 5μm） column was used with the mobile phase of acetonitrile （A） - 0. 2% （B） phosphate acid （0 -20min, A： B =2： 98; 20 -22min, A： B =2： 98--A： B = 11 ： 89） ; the flow rate was 1.2 mL · min^-1 , column temperature was at 30℃ and detection wavelength was at 220nm. Result： Gastrodia element and turpentine alcohot - biglucosid were linear in the range of 19. 3 - 193.21~g （ r = 0. 9997）, 6. 12 - 61.21xg （ r = 0. 9999） respectively. The average recovery of gastrodia element and turpentine alcohot - biglucosid were 100. 2%, 98. 5% respectively. Conclusion： This method was simple and accurate. It can be used in determination of gastrodia element and turpentine alcohot - biglucosid in Chinese medicine compound formulations Gastrodia and lJncaria Drink.