喉癌中HPV不同亚型的检测及测序 |
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引用本文: | 彭方毅,姜海蓉,刘芳,杜志银,林治华,彭方亮,赵卫兵. 喉癌中HPV不同亚型的检测及测序[J]. 重庆医科大学学报, 2009, 34(12) |
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作者姓名: | 彭方毅 姜海蓉 刘芳 杜志银 林治华 彭方亮 赵卫兵 |
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作者单位: | 重庆理工大学化学与生物工程学院制药工程系,重庆,400050;重庆市江北区第一人民医院检验科,重庆,400020;重庆医科大学图书馆文献检索教研室,重庆,400016;重庆市第四人民医院妇产科,重庆,400014;重庆市第四人民医院麻醉科,重庆,400030 |
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基金项目: | 国家自然科学基金资助项目,国家自然科学基金重点项目,重庆市科委自然基金,重庆市教委自然基金 |
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摘 要: | 目的:检测人乳头瘤病毒(Human papillomavirus,HPV)各型DNA在喉癌组织中的表达,探讨HPV在喉癌发生中的作用.方法:用PCR检测123例喉癌组织及123例喉粘膜上皮组织中HPV各型DNA.同时进行DNA测序,结果:在123例喉癌标本中,HPV-L1,-16E6/E7,-18E6/E7阳性率都明显高于其他各型.123例喉粘膜PCR结果表明:低危型HPV的阳性率与喉癌相近;高危型HPV的阳性率虽远远低于喉癌,但随着组织病变程度加重阳性率逐渐升高;HPV-L1的阳性率高于HPV-16E6、E7,随着组织病变程度加重,HPV-L1的检出率逐渐增高.结论:HPV-16感染会增强喉上皮病变的风险,而HPV-18有协同作用;L1片段适合用于检测HPV感染.
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关 键 词: | 人乳头瘤病毒 聚合酶链反应 序列测定 喉癌 |
Detection and sequencing of different subtypes of HPV in laryngeal carcinoma |
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Abstract: | Objective:To look for the further evidence for different subtypes of HPV as carcinogenic factors in laryngeal carcinoma.Methods: We examined representative numbers of specimens from laryngeal cancer with highly sensitive PCR technique and sequencing for the presence of different subtypes of HPV. Results: The positive rates of HPV-L1, -16E6/E7, -18E6/E7 were significantly higher than other types in 123 cases of laryngeal carcinoma specimens. PCR was used to screen the prevalence of HPV infection. The prevalence of HPV-16E6 was lower than laryngeal carcinoma. But, as the cells undergoing pathological progress, the HPV-16E6 positive rate was increased. The prevalence of HPV-16E7 was similar to the prevalence of HPV-16E6. The positive rate of HPV-L1 was the highest and the prevalence of HPV-L1 was significantly increased as the cells underwent pathological progress.Conclusion: HPV-16 and HPV-18 may synergistically function on the pathogenesis of laryngeal epithelium. L1 segment is fit for detecting HPV infection. |
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Keywords: | HPV Polymerase chain reaction Sequencing Laryngeal carcinoma |
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