喉癌中HPV不同亚型的检测及测序

目的:检测人乳头瘤病毒(Human papillomavirus,HPV)各型DNA在喉癌组织中的表达,探讨HPV在喉癌发生中的作用.方法:用PCR检测123例喉癌组织及123例喉粘膜上皮组织中HPV各型DNA.同时进行DNA测序,结果:在123例喉癌标本中,HPV-L1,-16E6/E7,-18E6/E7阳性率都明显高于其他各型.123例喉粘膜PCR结果表明:低危型HPV的阳性率与喉癌相近;高危型HPV的阳性率虽远远低于喉癌,但随着组织病变程度加重阳性率逐渐升高;HPV-L1的阳性率高于HPV-16E6、E7,随着组织病变程度加重,HPV-L1的检出率逐渐增高.结论:HPV-16感染会增强喉上皮病变的风险,而HPV-18有协同作用;L1片段适合用于检测HPV感染.

Detection and sequencing of different subtypes of HPV in laryngeal carcinoma

Objective:To look for the further evidence for different subtypes of HPV as carcinogenic factors in laryngeal carcinoma.Methods: We examined representative numbers of specimens from laryngeal cancer with highly sensitive PCR technique and sequencing for the presence of different subtypes of HPV. Results: The positive rates of HPV-L1, -16E6/E7, -18E6/E7 were significantly higher than other types in 123 cases of laryngeal carcinoma specimens. PCR was used to screen the prevalence of HPV infection. The prevalence of HPV-16E6 was lower than laryngeal carcinoma. But, as the cells undergoing pathological progress, the HPV-16E6 positive rate was increased. The prevalence of HPV-16E7 was similar to the prevalence of HPV-16E6. The positive rate of HPV-L1 was the highest and the prevalence of HPV-L1 was significantly increased as the cells underwent pathological progress.Conclusion: HPV-16 and HPV-18 may synergistically function on the pathogenesis of laryngeal epithelium. L1 segment is fit for detecting HPV infection.