| 柱前衍生-反相高效液相色谱法测定转移因子胶囊中游离氨基酸 |
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| 引用本文： | 刘海涛,张润婕,吴艳,王亚南,车慧. 柱前衍生-反相高效液相色谱法测定转移因子胶囊中游离氨基酸[J]. 中国生化药物杂志, 2007, 28(4): 233-235 |
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| 作者姓名： | 刘海涛 张润婕 吴艳 王亚南 车慧 |
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| 作者单位： | 总后勤部,卫生部,药品仪器检验所,北京,100071 |
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| 摘 要： | 目的用柱前衍生-反相高效液相色谱法测定转移因子胶囊中游离氨基酸的含量。方法采用二硝基氯苯(DNCB)为衍生试剂进行衍生,Waters Novapak C8(3.9 mm×150 mm,4μm)色谱柱分离,以乙腈-乙酸钠水溶液为流动相,梯度洗脱,检测波长360 nm,在65 min内,测定各氨基酸含量。结果回收率为97.5%~103.5%,RSD为0.7%~3.0%。结论DNCB柱前衍生-反相高效液相色谱法可测定转移因子胶囊中游离氨基酸的含量。
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| 关 键 词： | 反相高效液相色谱法 柱前衍生 游离氨基酸 转移因子胶囊 |
| 文章编号： | 1005-1678（2007）04-0233-03 |
| 收稿时间： | 2006-09-11 |
| 修稿时间： | 2006-09-112006-10-23 |
Determination of free amino acids in transfer factor capsules by pre-column derivatization with RP-HPLC |
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| LIU Hai-tao,ZHANG Run-jie,WU Yan,WANG Ya-nan,CHE Hui. Determination of free amino acids in transfer factor capsules by pre-column derivatization with RP-HPLC[J]. Chinese Journal of Biochemical Pharmaceutics, 2007, 28(4): 233-235 |
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| Authors: | LIU Hai-tao ZHANG Run-jie WU Yan WANG Ya-nan CHE Hui |
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| Abstract: | Purpose A pre-column derivatization with RP-HPLC method was established for the determination of content of free amino acids in transfer factor capsules.Methods DNCB was used as derivatization reagent,acetonitrile-sodium acetate solution as mobile phase,and detection wavelength was 360 nm.Results The average recovery was 98.1%-101.5% and RSD was 0.9%-3.3%.Conclusion It could be used for determination of content of free amino acids in transfer factor capsules. |
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| Keywords: | RH-HPLC pre-column derivatization free amino acids transfer factor capsules |
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