基质金属蛋白酶及其组织抑制物在子痫前期患者胎盘中的基因表达

目的研究子痫前期患者胎盘组织中基质金属蛋白酶(MMP-9、MMP-2)及其组织抑制物(TIMP-1、TIMP-2)基因mRNA表达,探讨其与子痫前期发病、进展的关系.方法采用半定量RT-PCR方法对30例正常晚期妊娠和50例子痫前期患者(包括24例轻度和26例重度)胎盘组织中MMP-2、MMP-9、TIMP-1和TIMP-2的mRNA水平进行检测.结果子痫前期组胎盘组织的MMP-9基因表达水平显著下降(P＜0.01),重度子痫前期组MMP-9基因表达水平尤低(0.07±0.05).尽管随病情的加重MMP-2mRNA水平呈现逐渐下降趋势,但没有统计学差异(P＞0.05).子痫前期组胎盘组织中的TIMP-1mRNA水平明显高于正常晚期妊娠者(P＜0.01),重度子痫前期组更高(1.49±0.21).子痫前期组胎盘组织TIMP-2mRNA表达显著降低(1.14±0.36),P＜0.01),并有随病情加重逐渐下降的趋势.结论随子痫前期患者病情的加重,胎盘组织的促浸润基因(MMP-2和MMP-9)表达水平降低,抑侵润基因(TIMP-1)的表达水平升高.子痫前期患者浸润相关基因的表达水平与子痫前期病情密切相关.

mRNA expression of MMPs and TIMPs in placenta of preeclampsia

Objective] To investigate the mRNA expressions of matrix metalloproteinase(MMP)-2,-9 and tissue inhibitior of metalloproteinase (TIMP)-1,-2 in placental tissue of patients with preeclampsia and their relation to the pathogenesis and development of preeclampsia. Methods] Semi-quantitative RT-PCR analysis was used to detect the MMP-2, MMP-9, TIMP-1, TIMP-2mRNA expression levels in placental tissue of 50 cases of patients with preeclampsia ( 24 cases of mild and 26 cases of severe preeclampsia) and 30 cases of normalterm pregnant women.Results] The MMP-9 gene expression level was significantly reduced in placental tissue of preeclampsia (P ＜0.01),which was the lowest in those of severe preeclampsia(0.07±0.05). With the development of preeclampsia, the MMP-2 gene expression level appeared a descent tendency without significance(P＞0.05). The expression of TIMP-1 mRNA were significantly higher in the placental tissue of severe preeclampsia patients than those of normal term (P ＜0.01),which was the highest in those of severe preeclampsia (1.49±0.21). The TIMP-2 gene expression level was significantly reduced in placental tissue of preeclampsia (1.14±0.36, P ＜0.01), which appeared a descent tendency with the severity of preeclampsia. Conclusions] The expression of invasion related gene (MMP-2 and MMP-9) mRNA reduced and invasion suppressor gene (TIMP-1) mRNA increased with the development of preeclampsia. The expression of invasion related gene mRNA correlated with the severity of preeclampsia.