独山瓜馥木总生物碱抗结核活性初探

目的系统评价独山瓜馥木二氯甲烷提取物3种组分的抗结核作用。方法采用抗结核活性跟踪法,从独山瓜馥木根中分步提取具有抗结核活性的组分,并采用Alarmar-Blue液体药敏法进行体外抗结核活性的评价。结果从独山瓜馥木根中获得了具有抗结核活性的二氯甲烷提取物(组分1)。薄层层析定性分析为总生物碱,进一步分离纯化得到组分2和3。药敏结果显示组分1和组分2对异烟肼和利福平的单耐药及耐多药结核分枝杆菌均有明显的抑制作用,尤其是组分2,对3/4的耐多药菌株的最小抑菌浓度小于40μg/mL。结论在当前耐药结核病流行的严峻形势下,独山瓜馥木提取物有治疗单耐和耐多药结核病的研发前景,其有效成分及抗结核机制有待进一步研究。

In vitro anti-tuberculosis activity of the extracts of the Fissistigma cavaleriei root

In order to explore the anti-tuberculosis activity of the extracts of Fissistigma cavaleriei root. Components extracted from the root of Fissistigma cavaleriei by the bioactivity-guided isolation method were tested by in vitro Alarmar-Blue drug sensitivity test of mycobacteria. Three components were separated and purified, in which component 1 and 2 could effectively inhibit the growth of the rifampicin-resistant and multi-drug resistant Mycobacteria strains. The anti-tuberculosis activity of component 2 was higher than that of other components, with the MIC value of 3/4 multi-drug resistant strains lower than 40 μg/mL. Under the current severe situation of the prevalence of drug-resistant tuberculosis, the extracts of Fissistigma cavaleriei have potential in the drug development of mono- and multidrug-resistant tuberculosis. Its effective components and the mechanism of anti-tuberculosis need further study.