呼肠孤病毒空斑检测方法建立

目的 建立呼肠孤病毒空斑检测方法,为进一步研究该病毒奠定基础。方法 通过Western Blot、免疫荧光检测纳尔逊海湾正呼肠孤病毒Miyazaki病毒株感染L929细胞和BSR细胞情况,病毒RNA水平凝胶电泳验证Miyazaki病毒株核酸成分。建立呼肠孤病毒空斑检测方法,计算病毒滴度。结果 Miyazaki病毒株能成功感染L929细胞和BSR细胞,水平凝胶电泳验证病毒株核酸成分正确,病毒株感染的L929细胞出现空斑,计算得出病毒滴度结果为2.2×10⁸ PFU/mL。结论 呼肠孤病毒空斑检测方法成功建立,该方法可以形成清晰可见的空斑,能稳定地对病毒滴度进行定量测定,为进一步研究呼肠孤病毒提供帮助。

Establishment of detection method for reovirus plaque

To establish a plaque detection method for reovirus, which laid the foundation for further study of the virus. Western blot and immunofluorescence were used to detect the infection of L929 cells and BSR cells with the Miyazaki virus strain of Nelson’s orthovirus, and the nucleic acid composition of the Miyazaki virus strain was verified by viral RNA horizontal gel electrophoresis. Establish a plaque detection method for reovirus and calculate the virus titer. The Miyazaki virus strain could successfully infect L929 cells. Horizontal gel electrophoresis verified that the nucleic acid composition of the virus strain was correct. L929 cells infected by the virus strain had plaques. The virus titer was calculated to be 2.2×10⁸ PFU/mL. The detection method of reovirus plaque was successfully established. This method can form clear and visible plaques, which can quantitatively determine the virus titer stably and provide help for the further study of reovirus.