饶河地区野生动物携带病毒的基因分型及序列特征分析

目的 确定当前饶河地区宿主动物所携带病原体的基因型别,分析其变异情况,对该地区自然宿主动物的感染情况进行监测。方法 使用TRIZOL法提取待测样本RNA,经RT-PCR扩增后对PCR产物进行核苷酸序列测定。应用DNAStar软件包对核苷酸序列进行同源性比较,构建系统进化树和氨基酸序列的比对。结果 在饶河地区,除褐家鼠(Rattus norvegicus)以外,松鼠(Sciurus vulgaris)也携带SEOV。本次从3只褐家鼠和1只松鼠体内分离出汉城型汉坦病毒(Seoul virus, SEOV),4株SEOV的遗传距离范围为0.00~0.01,与黑龙江地区常见SEOV病毒株亲缘关系较近。结论 黑龙江地区的气候和自然地理条件非常适合携带汉坦病毒(HV)宿主动物的生存和繁殖,但是随着人们生活条件的改善,禁止伐木狩猎,家鼠和野鼠与人类交集的逐渐减小,肾综合征出血热(HFRS)在黑龙江省暴发的概率也越来越小。

Genotyping and sequence analysis of wild animals carrying viruses in Raohe area,China

Raohe County,Heilongjiang Province is located at the Sino-Russian border.It is an important port of Sino-Russian trade,which is rich in animals species diversity.In this study,some wild animals in this area were screened for Flavivirus,Arenavirusgenus and Hantavirus(HV)pathogens.Nucleotide sequence of the gene fragments of the positive samples were analyzed.The results showed that Rattus norvegicus and Sciurus vulgaris carried SEOV in Raohe city.Through homology comparison,the genetic distance of the 4 SEOV ranges from 0.00 to 0.01,which was close to the common SEOV strain in Heilongjiang Province.The climate and natural geography of Heilongjiang are very suitable for the survival and reproduction of HV carrying host animals.But as people's living conditions improve,Prohibit logging and hunting,House mice and wild mice gradually reduce the intersection of humans,The probability of HFRS outbreak in Heilongjiang Province is also getting smaller and smaller.