促性腺激素在促排卵周期未成熟卵母细胞体外培养中的应用

目的 评价卵泡刺激素和人绒毛膜促性腺激素在促排卵周期未成熟卵母细胞体外培养过程中的应用价值。方法 收集控制性超促排卵周期行单精子显微注射(ICSI)过程中得到的未成熟卵母细胞(MⅠ期和GV期), 随机分为加促性腺激素培养组和不加促性腺激素培养组, 培养24~28 h后成熟卵母细胞进行ICSI授精, 并观察胚胎发育情况, 记录成熟率、受精率、卵裂率和优胚率。结果 对于MⅠ期卵母细胞, 是否使用促性腺激素不影响其24 h成熟率、受精率、分裂率和优胚率(P>0.05)。对于GV期卵母细胞, 培养液中加入促性腺激素能提高优质胚胎率(31.3% vs 4.2%, P<0.05), 但两组的成熟率、受精率和卵裂率差异无统计学意义(P>0.05)。结论 控制性超促排卵中获得的未成熟卵母细胞进一步体外培养, 无论是否添加促性腺激素都可自然成熟, 在GV期卵母细胞的体外培养过程加入促性腺激素可获得更高的优胚率。

Effect of Gonadotrophin on the in vitro maturation of immature oocytes from controlled ovarian stimulation

Objective To evaluate the effect of Follicle-stimulating hormone (FSH) and Human chrorionic gonadotrophin (HCG) on in vitro maturation of immature oocytes obtained from controlled ovarian stimulation (COH) cycles. Methods MⅠ and GV stage oocytes were collected from COH cycles for intracytoplasmic sperm injection (ICSI) treatment. All of these immature oocytes were divided into two groups (donadotrophin treatment group and non-donadotrophin treatment group) and cultured for 24 to 28 hours in insemination media. The donadotrophin group was treated with 0.075 IU/mL FSH and 0.15 IU/mL HCG. Matured oocytes were given ICSI. The rates of maturation, normal fertilization, cleavage, and high-quality embryo were recorded and compared. Results For MⅠ oocytes, there were no difference between the two groups in maturation rate, fertilization rate, cleavage rate and high-quality embryo rate (P>0.05). For GV oocytes, high-quality embryo rate in donadotrophin treatment group was higher than that in non-donadotrophin treatment group (31.3% vs 4.2%, P<0.05). Conclusion Immature oocytes from COH can mature in vitro whether hormones are added or not. During the maturation of the GV stage oocytes, donadotrophin can increasethe developmental potential of oocytes.