| 单克隆抗体双抗体夹心ELISA检测日本血吸虫病人循环抗原 |
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| 引用本文： | 龚红,李雍龙,韩家俊,王大坤.单克隆抗体双抗体夹心ELISA检测日本血吸虫病人循环抗原[J].寄生虫病与感染性疾病,1995(1). |
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| 作者姓名： | 龚红 李雍龙 韩家俊 王大坤 |
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| 作者单位： | 同济医科大学基础医学院寄生虫学教研室，武汉市生物制品研究所 |
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| 摘 要： | 本文用抗日本血吸虫肠相关抗原的单克隆抗体Ｓｊ１１６和Ｓｊ１０ｃ作双夹心ＥＬＩＳＡ检测粪检阳性的血吸虫病人血清中的循环抗原，阳性率达９３．４％。正常人假阳性率为６．７％。与肺吸虫、华支睾吸虫、旋毛虫及囊虫病的交叉反应分另伪３．２％、０、１３．３％和１３．３％。
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| 关 键 词： | 单克隆抗体，日本血吸虫，循环抗原，ELISA |
DETECTION OF CIRCULATING ANTIGEN IN PATIENTS WITH SCHISTOSOMIASIS JAPONICA BY DOUBLE ANTIBODY SANDWICH ELISA WITH MONOCLONAL ANTIBODY |
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| Gong Hong,Li Yonglong,Wang Dakun,Han Jiajun.DETECTION OF CIRCULATING ANTIGEN IN PATIENTS WITH SCHISTOSOMIASIS JAPONICA BY DOUBLE ANTIBODY SANDWICH ELISA WITH MONOCLONAL ANTIBODY[J].Parastoses and Infectious Diseases,1995(1). |
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| Authors: | Gong Hong Li Yonglong Wang Dakun Han Jiajun |
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| Abstract: | Circulating antigen was detected by double antibody sandwich ELISA with monoclonal antibodies Sj116 and Sj10c against Schistosoma japonicum gut-associated antigen.93.4% of patients with schistosomiasis japonica confirmed by feeal examination were positive in this assay.The false positive rate of sera from normal people was 6.7%.The rates of cross reaction with paragonimiasis, clonorchiasis,trichiniasis and cysticercosis patients were 3.2%,0.13.3% and 13.3% respectively. |
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| Keywords: | Monoclonal antibody Schistosoma japonicum circulationg antigen ELISA |
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