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| 藏药忍冬果对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子的影响 | |
| 引用本文： | 王聚乐,孙洋,周惠英,徐强,顿珠. 藏药忍冬果对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子的影响[J]. 中国中药杂志, 2006, 31(2): 145-148 |
| 作者姓名： | 王聚乐 孙洋 周惠英 徐强 顿珠 |
| 作者单位： | 1. 西藏大学,医学院,西藏,拉萨,850000 2. 南京大学,生命科学学院,生物医药技术国家重点实验室,江苏,南京,210093 3. 西藏自治区藏医学院,西藏,拉萨,850000 |
| 摘 要： | 目的:研究藏药忍冬果水提物(FLM)对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子的影响。方法:运用吞噬中性红试验观察FLM对小鼠腹腔巨噬细胞吞噬功能的影响,用小鼠腹腔巨噬细胞分泌物对小鼠胸腺细胞增殖及对L929细胞杀伤作用的影响分别测定IL-1及TNF-α的活性,用RT-PCR技术观察药物对小鼠腹腔巨噬细胞TNF-αmRNA和IFN-γmRNA表达的影响。结果:FLM在1～100μg.mL-1剂量内对小鼠腹腔巨噬细胞吞噬功能有明显促进作用,同时能诱导巨噬细胞分泌IL-1及TNF-α,并促进细胞因子TNF-αmRNA和IFN-γmRNA的表达。结论:FLM对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子都有促进作用。 |
| 关 键 词： | 忍冬果 巨噬细胞 吞噬作用 细胞因子 |
| 文章编号： | 1001-5302（2006）02-0145-04 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-05-08 |
Effects of traditional tibetan medicine,Fructus Lonicerae microphyllae on phagecytosis and cytokines production of murine macrophages |
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| WANG Ju-le ;SUN Yang ;ZHOU Hui-ying ;XU Qiang ;DUN Zhu. Effects of traditional tibetan medicine,Fructus Lonicerae microphyllae on phagecytosis and cytokines production of murine macrophages[J]. China Journal of Chinese Materia Medica, 2006, 31(2): 145-148 | |
| Authors: | WANG Ju-le SUN Yang ZHOU Hui-ying XU Qiang DUN Zhu |
| Affiliation: | Department of Pharmacology, School of Medicine, Tibet University, Lasa 850000, China. jule-wang@163.com |
| Abstract: | OBJECTIVE: To explore the effects of traditional Tibetan medicine, Fructus Lonicerae microphyllae (FLM) on phagecytosis and cytokines production of murine macrophages. METHOD: The phagecytosis of murine macrophages was analyzed by neutral red phagecytosis assay. The activities of IL-1 and TNF-alpha were measured by biological methods. The mRNA of TNF-alpha and INF-gamma expressed by macrophages was detected by RT-PCR. RESULT: The phagecytosis of murine macrophages was significantly enhanced by FLM at a concentration from 1 microg x mL(-1) to 100 microg x mL(-1) and the secretions of IL-1, and TNF-alpha from macrophages were markedly induced by FLM. Meanwhile, FLM also increased the expression of TNF-alpha mRNA and INF-gamma mRHA from macrophages in vitro. CONCLUSION: FLM could promote phagecytosis and cytokines production of murine macrophages. |
| Keywords: | Fructus Lonicerae microphyllae macrophage phagecytosis cytokine |
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