p27kip1和ki-67在寻常性银屑病皮损中的表达

目的检测p27kip1和ki-67在寻常性银屑病皮损中的表达水平,探讨其在银屑病发病中的意义。方法用免疫组化SP法检测细胞周期素依赖性蛋白激酶抑制剂p27kip1和增殖细胞核抗原ki-67在寻常性银屑病皮损及正常人皮肤中的表达水平。结果慢性斑块状与滴状皮损中p27kip1表达水平均显著低于正常人对照组(P<0.05和0.001),而慢性斑块状与滴状差异无统计学意义(P>0.05)。慢性斑块状与滴状皮损中ki-67表达水平均显著高于正常人对照组(P均<0.01);慢性斑块状皮损中ki-67表达水平低于滴状(P<0.01)。在寻常性银屑病皮损中,p27kip1表达和ki-67表达未发现相关性(r=0.08,P>0.05)。结论银屑病的发生可能与p27kip1下调有关;而ki-67的表达水平反映了角质形成细胞增殖状态。

Effects of p27kipl and ki-67 in Patients with Psoriasis Vulgaris

Objective To investigate the levels of p27kip1 and ki-67 of psoriatic lesions in patients with psoriasis vulgaris. Methods The expression of p27kip1 and ki-67 in psoriatic lesions was determined by immunohistochemistry. Results The level of p27kip1 expression was significantly lower in the plaque-form lesions of the patients with psoriasis than that in the normal controls(P < 0.05). The expression level of p27kip1 significantly decreased in the guttate lesions of the patients with psoriasis compared with the normal controls (P < 0.001). Between the guttate and plaque-form lesions, there was no significant difference in the expression level of p27kip1 (P > 0.05). The expression level of ki-67 was significantly higher in plaque-form lesions than that in the normal controls(P < 0.01). The expression level of ki-67 significantly increased in guttate lesions compared with the normal controls(P < 0.001). The expression level of ki-67 was significantly higher in guttate lesions than that of the plaque-form lesions (P < 0.001). There was no correlation between the expression level of p27kip1 and ki-67 in psoriatic lesions (r = 0.08). Conclusions The down-regulation of p27kip1 may be involved in the pathogenesis of psoriasis vulgaris. And the expression level of ki-67 may reflect the overproliferation of keratinocytes in psoriasis vulgaris.