| GPIb是血小板膜上一种重要的凝血酶受体 |
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| 引用本文： | 李海乾,邵波静,阮长耿. GPIb是血小板膜上一种重要的凝血酶受体[J]. 中国血液流变学杂志, 2002, 12(1): 1-5 |
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| 作者姓名： | 李海乾 邵波静 阮长耿 |
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| 作者单位： | 江苏省血液研究所,苏州大学附属第一医院血栓室,苏州,215006 |
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| 基金项目： | 国家自然科学基金资助课题 ( 39830 180 ) |
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| 摘 要： | 目的 研究GPIb在凝血酶诱导的血小板活化过程中的作用。方法 FCM法检测SZ-16的组织特异性，比浊法检测该单抗对血小板聚集的抑制作用。放射免疫法检测SZ-16对凝血酶诱导的血小板活化的影响。结果 SZ-16只与血小板结合，不与红细胞，白细胞和内皮细胞结合；血小板聚集实验表明，SZ-16对凝血酶诱导的血小板聚集有抑制作用；放射免疫结果表明，SZ-16抑制凝血酶引起的GMP140释放和TXB2的产生。结论 GPIb是血小板上的一种重要的凝血酶受体。
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| 关 键 词： | GPIb 血小板膜 凝血酶受体 单克隆抗体 血小板聚集 |
| 文章编号： | 1009-881X(2002)01-0001-05 |
| 修稿时间： | 2001-12-10 |
Glycoprotein Ib - a Major Thrombin Receptor on Platelet |
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| LI Hai-qian,SHAO Bo-jing,RUAN Chang-geng. Glycoprotein Ib - a Major Thrombin Receptor on Platelet[J]. Chinese Journal of Hemorheology, 2002, 12(1): 1-5 |
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| Authors: | LI Hai-qian SHAO Bo-jing RUAN Chang-geng |
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| Abstract: | Objective To Study the function of GPIb in platelet activation induced by thrombin.Methods FCM examines the specificity of SZ-16. Turbidimetry examines the antibody inhibitory effect on platelet aggregation by exogenous agonist. Effect of SZ-16 to platelet activation induced by thrombin is examined by radioimmunoasay.Results SZ-16 could only bind to platelet, not to RBC?WBC and EC.Aggregation test showed SZ-16 could inhibite thrombin-induced platelet aggregation, and had not influence on ADP?Ristocetin and AA-induced platelet aggregation. Radioimmunoassay test showed SZ-16 could inhibit GMP140 release and TXB2 production induced by thrombin.Conclusion GPIb is an important thrombin receptor on platelet. |
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| Keywords: | thrombin platelet monoclonal antibody |
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