| 当归多糖诱导人白血病干细胞衰老与调控端粒系统机制的研究 |
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| 引用本文： | 李成鹏,刘俊,贾道勇,张梦思,徐春燕,张岩岩,景鹏伟,王璐,王顺和,王亚平. 当归多糖诱导人白血病干细胞衰老与调控端粒系统机制的研究[J]. 中草药, 2014, 45(16): 2364-2369 |
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| 作者姓名： | 李成鹏 刘俊 贾道勇 张梦思 徐春燕 张岩岩 景鹏伟 王璐 王顺和 王亚平 |
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| 作者单位： | 重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 病理学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 病理学教研室, 重庆 400016;重庆医科大学 干细胞与组织工程研究室, 重庆 400016;重庆医科大学 组织学与胚胎学教研室, 重庆 400016 |
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| 基金项目： | 国家自然科学基金资助项目（81173398） |
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| 摘 要： | 目的探讨当归多糖(ASP)调控人CD34+CD38-骨髓白血病干细胞(LSC)衰老的端粒与端粒酶机制。方法免疫磁性分选人骨髓CD34+CD38-LSC;CCK-8检测ASP对CD34+CD38-LSC增殖抑制能力;衰老相关β半乳糖苷酶(SA-β-Gal)染色检测细胞衰老情况;混合集落培养(CFU-Mix)检测细胞增殖能力;荧光定量RT-PCR检测端粒酶基因TERT变化;TRAP-PCR检测细胞端粒酶活性;Southern blotting检测细胞端粒长度变化。结果免疫磁性分选后人骨髓CD34+CD38-LSC纯度达(91.15±2.41)%,相差显微镜观察显示细胞形态饱满,透明度高,折光性强。ASP体外诱导CD34+CD38-LSC 48 h,能有效抑制其增殖且呈浓度依赖性(P<0.05),集落形成能力下降(P<0.01)。40μg/mL ASP作用CD34+CD38-LSC 48 h,SA-β-Gal染色阳性细胞率明显升高(P<0.01);CD34+CD38-LSC端粒酶基因TERT表达水平下降(P<0.05);端粒酶活性下降(P<0.05);端粒长度缩短(P<0.05)。结论 ASP在体外可能通过调控细胞端粒系统诱导人骨髓CD34+CD38-LSC衰老。
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| 关 键 词： | 当归多糖 白血病干细胞 CD34+CD38-细胞 端粒 端粒酶 衰老 |
| 收稿时间： | 2013-12-23 |
Study on mechanism of telomere system regulation and senescence induction of leukemia stem cells by Angelica sinensis polysaccharide |
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| LI Cheng-peng,LIU Jun,JIA Dao-yong,ZHANG Meng-si,XU Chun-yan,ZHANG Yan-yan,JING Peng-wei,WANG Lu,WANG Shun-he and WANG Ya-ping. Study on mechanism of telomere system regulation and senescence induction of leukemia stem cells by Angelica sinensis polysaccharide[J]. Chinese Traditional and Herbal Drugs, 2014, 45(16): 2364-2369 |
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| Authors: | LI Cheng-peng LIU Jun JIA Dao-yong ZHANG Meng-si XU Chun-yan ZHANG Yan-yan JING Peng-wei WANG Lu WANG Shun-he WANG Ya-ping |
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| Affiliation: | Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Pathology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Pathology, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | |
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| Keywords: | Angelica sinensis polysaccharide leukemia stem cells CD34+CD38- cells telomere telomerase senescence |
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