青龙衣有效部位化学成分研究

目的研究青龙衣(核桃楸Juglans mandshurica的未成熟外果皮)有效部位的化学成分。方法采用正相硅胶柱色谱、Sephadex LH-20、高效液相制备色谱等方法进行分离纯化,并通过NMR等波谱鉴定化合物结构。结果从青龙衣95%乙醇提取物中分离得到15个化合物,分别鉴定为山柰酚(1)、齐墩果酸(2)、大黄酚(3)、乔松酮(4)、(2S)-5-羟基-6,7-二甲氧基二氢黄酮(5)、胡桃宁B(6)、左旋胡桃种萘醌(7)、绿原酸(8)、(4S)-4-羟基-1-四氢萘酮(9)、(4S,5S,7R,8R,14R)-8,11-二羟基-2,4-环桉叶烷(10)、5-羟基-3,7,3′,4′-四甲氧基黄酮(11)、(2S)-5,7,4′-三羟基二氢黄酮(12)、对羟基苯甲酸(13)、对甲氧基苯乙酸(14)、对苯二酚(15)。结论化合物8~15为首次从青龙衣中分离得到,其中化合物11、12、14、15为首次从胡桃属植物中分离得到。

Chemical constituents from active fraction in pericarps of Juglans mandshurica

Objective To study the chemical constituents from the effective fraction in pericarps of Juglans mandshurica. Methods The compounds were isolated and purified by various chromatographic methods, including silica gel, Sephadex LH-20, and preparative high performance liquid chromatography. Their structures were elucidated by NMR and MS analyses. Results Fifteen compounds were obtained and identified as kaempferol (1), oleanolic acid (2), chrysophanol (3), pinostrobin (4), (2S)-onysilin (5), juglanin B (6), (S)-regiolone (7), chlorogenic acid (8), (4S)-4-hydroxy-1-tetralone (9), (4S, 5S, 7R, 8R, 14R)-8, 11-dihydroxy-2, 4-cyclo-eudesmane (10), 5-hydroxy-3, 7, 3', 4'-tetramethoxyflavone (11), (2S)-5, 7, 4'-trihydroxy-dihydroflavonol (12), p-hydroxybenzonic acid (13), p-methoxyphenylacetic acid (14), and 1, 4-dihydroxybenzene (15). Conclusion Compounds 8-15 are first isolated from the pericarps of J.mandshurica and compounds 11, 12, 14, and 15 are obtained from the plants of Juglans L. for the first time.