高良姜素对氢醌诱导的白癜风小鼠模型的影响

目的研究不同质量分数高良姜素对氢醌诱导白癜风小鼠模型的影响。方法采用2.5%氢醌涂抹小鼠背部剃毛后的区域以诱导白癜风动物模型,分光光度法测定血清中的酪氨酸酶(TYR)、丙二醛(MDA)水平及胆碱酯酶(CHE)活性,分别采用HE染色法、多巴特殊染色法、Lillie染色法观察不同质量分数高良姜素对小鼠皮肤含黑色素毛囊数、基底层黑素细胞数和含黑素细胞表皮细胞数的影响;采用免疫组化法检测小鼠皮肤中TYR蛋白表达。结果模型组小鼠背部毛发颜色明显较对照组小鼠白,2.5%氢醌诱导后,模型小鼠含黑色素的毛囊数、基底层黑素细胞和含黑素颗粒表皮细胞数量明显较对照组低。经过高良姜素治疗后,小鼠背部毛发颜色由白变黑;与模型组相比,高良姜素治疗组的小鼠皮肤含黑色素毛囊数量明显上升(P<0.01);4.250 mg/kg 90%高良姜素组基底层黑素细胞数和含黑素颗粒表皮细胞数显著上升(P<0.05、0.01),皮肤TYR蛋白表达也显著升高(P<0.05);0.425、4.250 mg/kg 90%高良姜素能够显著升高小鼠血清TYR水平,降低MDA水平及CHE活性(P<0.05、0.01);4.250、42.500 mg/kg 99%高良姜素显著提高基底层黑素细胞数(P<0.01);4.250 mg/kg99%高良姜素能够显著提高含黑素颗粒表皮细胞数(P<0.05),降低血清CHE活性(P<0.01)。99%高良姜素3个剂量均能够升高血清TYR水平(P<0.05、0.01),0.425、4.250 mg/kg 99%高良姜素降低血清MDA水平(P<0.01)。结论不同质量分数的高良姜素均能够改善氢醌诱导的小鼠白癜风症状,其作用机制可能是提高TYR活性,降低机体的MDA和CHE水平,但二者的治疗效果及作用机制存在一定的差异。

Effects of galangin with different purity on vitiligo mouse model induced by hydroquinone

Objective To study the effects of galangin with different purity on vitiligo mouse model induced by hydroquinone. Methods The vitiligo mouse model was induced by smearing 2.5% hydroquinone at shaving areas on dorsal skin for each mouse. The concentration of tyrosinase (TYR), the content of malondialdehyde (MDA), and the activity of cholinesterase (CHE) in serum were measured spectrophotometrically. The melanin-containing hair follicles, basal melanocytes, and melanin-containing epidermal cells were measured by histological analysis. The expression of the TYR protein in skin of mice was detected by immunohistochemical methods. Results Experimental results showed that the hair color of mice in model group was whiter than that of mice in control group, and the numbers of melanin-containing follicle, the numbers of basal melanocyte, and melanin-containing epidermal cell in dorsal skin of mice induced by hydroquinone (2.5%) (model group) were significantly decreased compared with the control group. The color of hair in dorsal skin of mice turned black from white after treatment of galangin with different purity. Histological analysis showed that the numbers of melanin-containing hair follicle in shaving areas were found to be increased at all dosages of the galangin groups (P < 0.01, compared with the model group). While the numbers of skin basal layer melanocyte and melanin-containing epidermal cell were significantly increased at the dosage of 4.25 mg/kg of the 90% galangin (P < 0.05, 0.01), and the expression of skin TYR protein was also increased; The concentration of TYR in serum was increased, the content of MDA and the activity of CHE were decreased at 0.425 and 4.250 mg/kg doses of 90% galangin groups (P < 0.05, compared with the model group). The numbers of skin basal layer melanocyte were increased at 4.250 and 42.500 mg/kg doses of 99% galangin groups; the numbers of melanin-containing epidermal cell were increased (P < 0.05), and the activity of CHE was decreased (P < 0.01) at 4.250 mg/kg dose of 99% galangin group; The concentration of TYR in serum was increased at all dosages of 99% galangin groups; The content of MDA was decreased at 0.425 and 4.250 mg/kg doses of 99% galangin groups (P < 0.01). Conclusion The result shows that galangin with different purity could improve vitiligo syndrome induced by hydroquinone in mice. The mechanism might be related to the concentration of TYR, the expression of TYR protein, the activity of MDA, and the content of CHE. But there are some differences between 90% galangin and 99% galangin in effects of treatment and mechanism. Galangin might become the one of potential candidate drugs for treating vitiligo diseases after more experiments have been carried out.