杀菌/通透性增加蛋白对内毒素休克大鼠微循环灌注的保护作用

目的 探讨杀菌／通透性增加蛋白（ＢＰＩ）对内毒素休克时血液动力学和内脏微徨灌注的保护作用及其机制。方法 采用大鼠内毒素休克模型，动态观察血液动力学、内脏微循环灌注量和血浆生物喋吟、一氧化氮（ＮＯ）水平的变化。结果 休克早期给予重组ＢＰＩ，可显著提高平均支流压、心脏指数及每搏输出量，明显改善肝、肾、小肠抽徨灌注量及动物预后（Ｐ〈０．０５～０．０１），循环ＮＯ产生亦不同程度受到抑制。结论 早期应用ＢＰ

The protective effects of recombinant amino terminal fragment of bactericidal/permeability increasing protein on systemic hemodynamics and microcirculation in a rat model of endotoxic shock

Objective To evaluate the potential effects of recombinant amino terminal fragment of bactericidal/permeability increasing protein(rBPI 21 )on systemic hemodynamics and internal organ microcirculation following septic shock.Methods Adult male rats were subjected to endotoxic shock induced by a bolus intravenous injection of endotoxin(10.0 mg/kg),and hemodynamic parameters,internal organ microvascular blood flow,plasma biopterin,as well as nitric oxide levels were determined in rats at various intervals.Results Early treatment with rBPI 21 significantly elevated arterial blood pressure,cardiac index,and stroke volume compared with non treated group(P<0 05 0 01) Meanwhile,microvascular blood flow in liver,kidney,as well as small intestine,and survival rate were markedly improved in animals received rBPI 21 treatment.In addition,plasma biopterin and nitric oxide levels were much lower in the treatment group than in the non treatment group (P<0 05 0 01).Conclusion During early stage after septic shock,administration of rBPI 21 can potently attenuate cardiovascular and microcirculatory dysfunction,which may be associated with its inhibitory effects on biopterin and nitric oxide synthesis evoked by endotoxin challenge.